Sequencing Analysis Viewer (SAV) Training

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  • Sequencing Analysis Viewer (SAV)

    This course provides the basics on how to get started using Sequencing Analysis Viewer (SAV). By the end of this course, you will be able to: identify the purpose of SAV, list the steps to load data into SAV, and describe the tabs in SAV.

    15 min


  • MiSeq: Does My Run Look Good?

    After completing this course you will be able to list two options for monitoring your MiSeq run, describe key metrics in MiSeq Control Software (MCS), and describe key metrics in Sequencing Analysis Viewer (SAV).

    10 min


  • MiniSeq: Does My Run Look Good?

    This course explores the options for monitoring your MiniSeq run. Also detailed are the key metrics in MiniSeq Control Software, Sequencing Analysis Viewer, and BaseSpace Sequence Hub.

    10 min


  • NextSeq: Does My Run Look Good?

    This course introduces four options for monitoring your NextSeq run. It also describes key metrics in NextSeq Control Software (NCS), Local Run Manager, Sequencing Analysis Viewer (SAV), and BaseSpace Sequence Hub.

    10 min


  • iSeq 100: Does My Run Look Good?

    This course discusses general guidelines for assessing your run’s performance.

    10 min


  • Is My HiSeq or MiSeq Run Overclustered?

    This video provides an overview of overclustering and how it can impact your sequencing data. An Illumina Field Applications Scientist shows you how to use Sequencing Analysis Viewer (SAV) to look for common symptoms of an overclustered HiSeq or MiSeq flow cell.

    7 min


  • How Can I Tell If I Sequenced Through the Insert? Part 1

    An Illumina Field Applications Scientist describes what it means to sequence through the insert of your prepared library. We look at Bioanalyzer traces to assess the range of insert sizes in a library.

    7 min


  • How Can I Tell If I Sequenced Through the Insert? Part 2

    This video shows you how to use Sequencing Analysis Viewer (SAV) plots to determine if your sequencing insert is too short. We also explore how to use the adaptertrimming.txt file on the MiSeq to diagnose short inserts.

    5 min


  • Sequencing Analysis Viewer (SAV): A Beginner's Guide

    Recorded Webinar (November 2019) | The Sequencing Analysis Viewer (SAV) Software is an application where users can view important quality metrics generated during sequencing runs. This webinar will provide a guided tour for beginners on how to use SAV, as well as tips and tricks for reviewing the most useful information for sequencing runs. This webinar is targeted at new users and will go over the following topics: how to load data into SAV, what metrics are available in each tab of the software, and understanding which are the most valuable metrics for run review and where to find them.


  • Sequencing Considerations for Low Diversity Libraries

    Recorded Webinar (July 2019) | Low diversity libraries, such as those used for amplicon sequencing, can present challenges on Illumina sequencing systems and may skew software performance and data accuracy resulting in poor quality runs. This webinar is ideal for new and intermediate users interested in considerations for the sequencing of low diversity libraries and will cover the following topics: Understanding and visualizing base diversity in Sequencing Analysis Viewer (SAV), strategies to optimize low diversity sequencing data, % PhiX spike-in and cluster density recommendations for different sequencing platforms, and an overview of base calling and quality score calculations.


  • Sequencing Considerations for Low Diversity Libraries

    Upcoming Webinar | Low diversity libraries, such as those used for amplicon sequencing, can present challenges on Illumina sequencing systems and may skew software performance and data accuracy resulting in poor quality runs. This webinar is ideal for new and intermediate users interested in considerations for the sequencing of low diversity libraries and will cover the following topics: understanding and visualizing base diversity in Sequencing Analysis Viewer (SAV), strategies to optimize low diversity sequencing data, % PhiX spike-in and cluster density recommendations for different sequencing platforms, and an overview of base calling and quality score calculations.

    Jan 23, 2020 PST