NextSeq 1000/2000 XLEAP-SBS kits are compatible with any third party index set, but when choosing an Illumina index set, please use Illumina DNA/RNA UD Indexes Sets A-D (20091654, 20091656, 20091658, 20091660).
Dilute pooled libraries to the appropriate concentration for sequencing.
Determine the best kit for your project type, starting material, and method or application.
Determine reagents and sequencing runs for your desired coverage.
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