Requirements & Compatibility

Kit Requirements

Sequencing Primers for Dual-Indexed Libraries

Sequencing primers provided in the TruSeq Dual Index Sequencing Primer Box are required for sequencing the following libraries on the HiSeq 1000:

  • Any Nextera libraries with any number of indices for single-read or paired-end runs. Use the single-read kit with single-read flow cells, and the paired-end kit with paired-end flow cells.
  • TruSeq HT libraries when performing a dual-indexed run on a single-read flow cell.

For instructions, see the HiSeq 1000 User Guide.

Kit and Software Versions

Cluster Kit Version

SBS Kit Version

Software Version

TruSeq Cluster Kit v3

TruSeq SBS Kit v3 

HCS v1.4 or later/RTA v1.12, or later


Software Version

TruSeq Dual Index Sequencing Primer Box

HCS v1.5 / RTA v1.13, or later