NextSeq 500/550 v2 kits have been discontinued. In addition, NextSeq TG reagents v1, v1.2, and v2 were discontinued on October 31, 2019, and the NextSeq TG v2.1 reagents were discontinued on July 31, 2019. With increased stability, the NextSeq 500/550 v2.5 Kits are the recommended replacement.
Performing a sequencing run requires a single-use NextSeq 500/550 Kit. Each kit contains a flow cell and reagent cartridge prefilled with reagents used for clustering the flow cell and performing the run.
The NextSeq 500/550 Kit (v1) requires manually adding sodium hypochlorite (NaOCl) and BP13 to the reagent cartridge before the run. For instructions, see Additional Setup Step for NextSeq 500 Kit v1. All other kits include dual-index sequencing primers and NaOCl in the prefilled cartridge. No additional steps are required.
For other system requirements and product compatibility, see Compatible Products.
Each reagent cartridge and flow cell is marked High or Mid for easy identification. Use the appropriate reagent cartridge for a given flow cell type. The buffer cartridge is universal.
|Kit Name||Flow Cell Type||Number of Tiles|
|NextSeq 500/550 High Output Kit
TG NextSeq 500 High Output Kit
|NextSeq high output flow cell||864 tiles|
|NextSeq 500/550 Mid Output Kit||NextSeq mid output flow cell||288 tiles|
Reagents are sensitive to light. Store the reagent and buffer cartridges in a dark location away from light.
|1||Reagent Cartridge||-25°C to -15°C||Contains clustering and sequencing reagents|
|1||Buffer Cartridge||15°C to 30°C||Contains buffers and wash solution|
|1||Flow Cell*||2°C to 8°C||Flow cell|
|1||HT1†||-25°C to -15°C||Hybridization Buffer|
* Shipped at room temperature. Store at 2°C - 8°C.
† HT1 dilutes libraries before sequencing. For more information, see the NextSeq System Denature and Dilute Libraries Guide.