NextSeq 500 Training

  • Introductory Topics

    DATE/LENGTH


  • NextSeq: System Overview

    By the end of this course, you will be able to: identify features of the NextSeq system, describe NextSeq sequencing technology, and describe the simplified sequencing workflow of the NextSeq system.

    20 min


  • Sequencing: Introduction to Sequencing By Synthesis (SBS)

    Recorded Webinar (July 2019) | Illumina next-generation sequencing technology allows for massive parallel sequencing. Illumina Technical Support invites you to join us for a presentation and discussion on sample preparation, cluster generation and sequencing by synthesis chemistry. This webinar is targeted to those new to next-generation sequencing and will discuss the following topics: Illumina library construction, cluster generation methods by platform, sequencing by synthesis, and multiplexing and primary analysis.


  • Sequencing: Introduction to Sequencing By Synthesis (SBS) - Brazil (Português)

    Upcoming Webinar | Quer aprender como tirar melhor proveito de suas corridas no Sistema MiSeq™? O suporte técnico da Illumina convida você para uma discussão sobre essa plataforma. O Sistema MiSeq permite que o usuário faça a amplificação, sequenciamento e análise de dados integrados em um único instrumento, com apenas alguns passos de configuração, e neste seminário via web ensinaremos como identificar possíveis problemas e soluções em cada uma dessas etapas. Este webinar é direcionado para novos e intermediários usuários com uma compreensão básica das etapas de sequenciamento da Illumina e do software Sequence Analysis Viewer (SAV). Atendendo à esse seminário você aprenderá: Solucionar problemas durante o preparo e início da corrida; Avaliar métricas de qualidade das corridas, em progresso e terminadas, usando SAV; Dicas para melhorar a qualidade do seu sequenciamento e boas práticas em um laboratório de biologia molecular.

    Nota: Apresentação oral às 2:00 pm (Brasilia Time)

    Nov 26, 2019 PST


  • Library Prep

    DATE/LENGTH


  • Illumina Experiment Manager

    This course provides an introduction to Illumina Experiment Manager, and shows you the steps to create sample plates and sample sheets.

    25 min


  • AmpliSeq for Illumina: Library Prep Protocol

    This course identifies the items needed for the AmpliSeq for Illumina library prep protocol, introduces pool planning resources for a multiplexed amplicon run, demonstrates the steps in the library prep protocol, and lists best practices for the protocol.

    30 min


  • AmpliSeq for Illumina: Overview

    This course describes the AmpliSeq for Illumina assay technology, lists the three types of panels, introduces the steps in the amplicon sequencing workflow, and lists the analysis options for amplicon sequencing data.

    15 min


  • Nextera DNA Flex Library Preparation

    By the end of this course, you will be able to identify the Nextera DNA Flex kit, describe how the protocol works, identify what is needed to complete the protocol, and define the various steps in the library preparation workflow.

    20 min


  • Instruments & Software

    DATE/LENGTH


  • IT Requirements for Implementing Illumina Proactive

    This video shares IT requirements for implementing Illumina Proactive, an instrument performance monitoring service provided by Illumina that can increase instrument uptime, improve operational efficiency, and reduce the risk of lost resources.

    4 min


  • Preparing Runs with BaseSpace Sequence Hub

    This course provides an overview of how to use the BaseSpace Sequence Hub Prep tab to enter sample information and parameters for your sequencing run.

    25 min


  • NextSeq: Does My Run Look Good?

    This course introduces four options for monitoring your NextSeq run. It also describes key metrics in NextSeq Control Software (NCS), Local Run Manager, Sequencing Analysis Viewer (SAV), and BaseSpace Sequence Hub.

    10 min


  • NextSeq: How to Start a Run

    By the end of this course, you will be able to: identify the steps to start a run on the NextSeq, describe how to prepare the reagent cartridge, and start a sequencing run using NCS.

    20 min


  • Local Run Manager

    This course shows you how to get started using the Local Run Manager software to set up your sequencing runs and view analysis results.

    Languages: French, Italian, German, SpanishChinese (Simplified)

    20 min


  • Data Analysis

    DATE/LENGTH


  • BaseSpace Sequence Hub: Introduction and Analysis Overview

    Recorded Webinar (September 2019) | llumina Technical Support invites you to learn about BaseSpace, Illumina’s cloud-based sequencing data analysis solution. In this presentation, we will discuss how to navigate the BaseSpace dashboard, general BaseSpace functionally, and tips and tricks for successful application and workflow utilization. We will cover the following topics: How to upload/view data in BaseSpace, discussion of major analysis workflows, general overview of how to analyze data on BaseSpace.

    Please note: BaseMount and BaseSpace CLI are alpha software releases; features and functionality may change over time, and support is directly through Illumina software development.


  • Local Run Manager 2.0 (Research Use Only): Introduction - On- and Off-Instrument

    Upcoming Webinar | Local Run Manager is a Windows-based software for Illumina benchtop sequencing data that includes local analysis options as well as run and user management. This webinar targets new and intermediate users of the software. It will provide an overview of the Research Use Only (RUO) version of Local Run Manager 2, including the off-instrument version and the versions bundled with the latest MiSeq Control Software, iSeq Control Software, and NextSeq Control Software. This webinar does not cover the Dx versions of the software. We will cover the following topics: Features of Local Run Manager 2, available versions and analysis options, how to set up runs and analyze data, interaction with on-instrument control software, and comparison to other Illumina analysis software.

    Dec 17, 2019 PST