Contents & Storage

The AmpliSeq for Illumina BRCA protocol requires AmpliSeq for Illumina BRCA Panel (Catalog # 20019168), AmpliSeq for Illumina Library PLUS (Catalog # 20019101, 20019102, or 20019103), and AmpliSeq for Illumina CD Indexes Set A (96 indexes, 96 samples) (Catalog # 20019105).  The AmpliSeq for Illumina Library PLUS Kit is available in 24-, 96-, and 384-reaction configurations.

The AmpliSeq for Illumina BRCA Panel is a 2 oligo pool panel for DNA samples. The following number of kits are required to prepare the appropriate DNA samples. One DNA sample* will prepare one library

Number of Samples

24 Samples

96 Samples 384 Samples
Number of DNA Libraries 24 DNA Libraries 96 DNA Libraries 384 DNA Libraries
AmpliSeq for Illumina BRCA Panel 1 4 16
AmpliSeq Library PLUS For Illumina 1 - 24 reaction kit (20019101) 1 - 96 reaction kit (20019102) 1 - 384 reaction kit (20019103)
AmpliSeq CD Set A (96 reaction, 96 indexes) 1 1 4
cDNA Synthesis - - -

*A sample is a specimen of nucleic acid from an individual source. 

†A library is nucleic acids from a sample after preparation and contains the required ends for sequencing. 

AmpliSeq for Illumina BRCA Panel, Store at -25°C to -15°C*

Quantity

Reagent

   
1 5X AmpliSeq BRCA Primer Pool 1    
1 5X AmpliSeq BRCA Primer Pool 2    

*Ships at room temperature, but must be stored at -25°C to -15°C

AmpliSeq Library PLUS for Illumina Contents, Store at -25°C to -15°C

 

Tube Quantity for Kit Configuration

24-reaction

96-reaction

384-reaction Reagent
1 4 16 1X Lib Amp Mix
1 1 4 10X Library Amp Primers
1 1 4 DNA Ligase
1 1 4 5X AmpliSeq HiFi Mix
1 1 4 FuPa Reagent
1 2 8 Low TE**
1 1 4 Switch Solution

**Can be stored at room temperature

AmpliSeq for Illumina CD Indexes Set A, Store at -25°C to -15°C*

Quantity

Description

1 AmpliSeq CD Indexes Set A plate (96 indexes, 96 samples)

*Ships at room temperature, but must be stored at -25°C to -15°C

AmpliSeq Library Equalizer for Illumina

Quantity

Description

Storage
1 Equalizer Beads 2°C to 8°C
1 Equalizer Capture 2°C to 8°C
1 Equalizer Elution Buffer 2°C to 8°C
1 Equalizer Wash Buffer 15°C to 30°C
1 Equalizer Primer 2°C to 8°C