AmpliSeq for Illumina Custom and Community Panels RNA Checklist 04/16/2018
AmpliSeq for Illumina Custom and Community Panels Reference Guide 04/16/2018
AmpliSeq for Illumina Custom and Community Panels Consumables & Equipment 04/16/2018

Kit Guide

AmpliSeq for Illumina Custom and Community Panels Reference Guide

Input Requirements

This protocol is optimized for 10 ng DNA or 10 ng RNA per pool. Use a fluorometric-based method to quantify input.

Panel Specifications

DNA Panel (2X Concentration)

Number of Amplicons per Panel	One Primer Pool	Two Primer Pools	Three Primer Pools	Four Primer Pools
≤ 96	5 tubes	2 × 5 tubes	3 × 5 tubes	4 × 5 tubes
> 96	20 tubes	2 × 20 tubes	3 × 20 tubes	4 × 20 tubes

RNA Panel (5X Concentration)

Number of Amplicons per Panel	One Primer Pool	Two Primer Pools
12-1200	3 tubes	2 × 3 tubes

ampliseq-custom-dna-panel-photo-library-kit

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