AmpliSeq for Illumina Custom and Community Panels Training

  • Title


  • AmpliSeq for Illumina: Overview

    This course describes the AmpliSeq for Illumina assay technology, lists the three types of panels, introduces the steps in the amplicon sequencing workflow, and lists the analysis options for amplicon sequencing data.

    15 min

  • AmpliSeq for Illumina: Library Prep Protocol

    This course identifies the items needed for the AmpliSeq for Illumina library prep protocol, introduces pool planning resources for a multiplexed amplicon run, demonstrates the steps in the library prep protocol, and lists best practices for the protocol.

    30 min

  • DesignStudio: AmpliSeq for Illumina Custom DNA Design
    After completing this course, you will be able to create an AmpliSeq for Illumina custom DNA panel in DesignStudio. This course provides an introduction to DesignStudio, guides you through the steps of creating a custom DNA panel, and provides tips and additional resources to get you started.

    15 min

  • How Do I Optimize Amplicon Sequencing Data? Part 1

    What are the impacts of sequencing amplicon and low diversity libraries on Illumina instruments? An Illumina Field Applications Scientist discusses considerations for designing and performing amplicon sequencing.

    6 min

  • How Do I Optimize Amplicon Sequencing Data? Part 2

    An Illumina Field Applications Scientist discusses best practices for sequencing amplicons on Illumina instruments with high-quality results. We use Sequencing Analysis Viewer (SAV) to compare key metrics of amplicon sequencing runs to a standard PhiX run.

    7 min

  • Preventing Contamination

    Many next-generation sequencing applications use the Polymerase Chain Reaction, or PCR, for DNA amplification. This video presents best practices to minimize the potential for PCR contamination in your experiment.

    10 min

  • AmpliSeq for Illumina: Introduction

    Recorded Webinar (March 2020) | This webinar introduces our line of amplicon products: AmpliSeq for Illumina- compatible with Illumina next-generation sequencing platforms. We will discuss the following topics: available fixed panels, library preparation workflow, the new Design Studio, and and overview of analysis options.

  • DesignStudio: Introduction

    Recorded Webinar (March 2020) | Have a targeted sequencing project in mind? This webinar is targeted toward new and intermediate users looking to design a custom targeted sequencing assay in DesignStudio. We wiill show you how to use the software, and share tips and tricks to create a design for AmpliSeq for Illumina, Nextera Rapid Capture, and TruSeq Targeted RNA Expression.