Before preparing libraries, record information about your samples using an option available for your sequencing system:
AmpliSeq for Illumina TCR beta-SR Panel is a highly multiplexed targeted resequencing panel intended for use with multiple sample types, including formalin-fixed paraffin-embedded (FFPE) tumor samples.
|Component||Number of Pools||Concentration|
|DNA and RNA||1||5X|
Completing the AmpliSeq for Illumina TCR beta-SR protocol requires the panel and the following library prep kit and index kit.
For each DNA and RNA sample, the AmpliSeq for Illumina TCR beta-SR Panel generates one DNA and one RNA library. A sample is a specimen of nucleic acid from one source, while a library is nucleic acids from a sample after preparation. Samples can be DNA, RNA, or paired. A library has the ends required for sequencing.
Preparing the appropriate number of samples requires the following number and combination of kits.
|Number of Samples||24 Samples||96 Samples||384 Samples|
|Number of Libraries||24 Libraries||96 Libraries||384 Libraries|
|AmpliSeq for Illumina TCR beta-SR Panel||1||5||16|
|AmpliSeq Library PLUS For Illumina||1 24-reaction kit (20019101)||1 96-reaction kit (20019102)||1 384-reaction kit (20019103)|
|AmpliSeq CD Set A (96 reaction, 96 indexes)||1||1||4|
|AmpliSeq UD Indexes for Illumina (24 Indexes, 24 Samples)||1||4||16|
|[RNA] cDNA Synthesis||1||1||4|
Sequence AmpliSeq for Illumina TCR beta-SR Panel libraries using the following systems and reagent kits.
|MiniSeq System||MiniSeq Mid Output Reagent Kit|
|MiniSeq High Output Reagent Kit|
|NextSeq System||NextSeq Mid Output v2 Kit|
|NextSeq High Output v2 Kit|