The Nextera Rapid Capture Exome and Expanded Exome Enrichment kits are packaged in various 8, 24, 48, 72, and 96 sample configurations. The Exome and Expanded Exome kits differ in the oligo contents of Box 4/Oligos box.
Reagent |
Storage |
Description |
SPB |
2° to 8°C |
Sample Purification Beads |
SMB |
2° to 8°C |
Streptavidin Magnetic Beads |
ET2 |
2° to 8°C |
Elute Target Buffer 2 |
ST |
15° to 30°C |
Stop Tagment Buffer |
Reagent |
Storage |
Description |
RSB |
-15° to -25°C |
Resuspension Buffer |
TDE1 |
-15° to -25°C |
Tagment DNA Enzyme |
TD |
-15° to -25°C |
Tagment DNA Buffer |
EWS |
-15° to -25°C |
Enrichment Wash Solution |
NLM |
-15° to -25°C |
Library Amp Mix |
EHB |
-15° to -25°C |
Enrichment Hybridization Buffer |
HP3 |
-15° to -25°C |
2N NaOH |
PPC |
-15° to -25°C |
PCR Primer Cocktail |
NEM |
-15° to -25°C |
Enrichment Amp Mix |
EE1 |
-15° to -25°C |
Enrichment Elution Buffer 1 |
Reagent |
Storage |
Description |
Index Primers |
-15° to -25°C |
E502 to E508* |
Index Primers |
-15° to -25°C |
N701 to N712* |
*The indices provided vary per Nextera Rapid Capture Enrichment Kit configuration.
The Nextera Rapid Capture Exome and Expanded Exome Enrichment kits contain one of the following, depending on the kit type.
Kit |
Reagent |
Storage |
Description |
Exome |
CEX |
-15° to -25°C |
Coding Exome Oligos |
Expanded Exome |
EEX |
-15° to -25°C |
Expanded Exome Oligos |
Storage |
Description |
15° to 30°C |
i7 Index Tube Caps, Orange |
15° to 30°C |
i5 Index Tube Caps, White |