By the end of this course, you will be able to identify the Nextera XT DNA kits, identify how the protocol works, describe what is needed to complete the protocol, and define the various steps in the library preparation workflow.
In this video, an Illumina Field Applications Scientist discusses the key steps in the Nextera library preparation process and provides some tips and tricks on how to achieve optimal results. Note that the best practices presented in this video do not apply to the Nextera™ DNA Flex Library Prep Kit.
What are the impacts of sequencing amplicon and low diversity libraries on Illumina instruments? An Illumina Field Applications Scientist discusses considerations for designing and performing amplicon sequencing.
An Illumina Field Applications Scientist discusses best practices for sequencing amplicons on Illumina instruments with high-quality results. We use Sequencing Analysis Viewer (SAV) to compare key metrics of amplicon sequencing runs to a standard PhiX run.
This course provides an introduction to Illumina Experiment Manager, and shows you the steps to create sample plates and sample sheets.
Many next-generation sequencing applications use the Polymerase Chain Reaction, or PCR, for DNA amplification. This video presents best practices to minimize the potential for PCR contamination in your experiment.
Recorded Webinar (August 2017) | This session provides an overview of amplicon sequencing, including library preparation methods. This webinar is targeted to new users and covers the following topics: what defines an amplicon library, kits and protocols for amplicon libraries, and amplicon sequencing runs and parameters.
Recorded Webinar (August 2016) | Join Illumina Technical Support for a presentation and discussion on the Nextera XT sample preparation process. This session reviews the following topics: Nextera XT protocol, best practices for critical steps, and a comparison of the Nextera XT kit to the Nextera DNA kit, focusing on the applications recommended with each kit.