Nextera XT DNA Library Prep Kit Training

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  • Nextera XT DNA Library Preparation

    By the end of this course, you will be able to identify the Nextera XT DNA kits, identify how the protocol works, describe what is needed to complete the protocol, and define the various steps in the library preparation workflow.

    20 min


  • Best Practices for Nextera Library Prep

    In this video, an Illumina Field Applications Scientist discusses the key steps in the Nextera library preparation process and provides some tips and tricks on how to achieve optimal results. Note that the best practices presented in this video do not apply to the Nextera™ DNA Flex Library Prep Kit.

    9 min


  • How Do I Optimize Amplicon Sequencing Data? Part 1

    What are the impacts of sequencing amplicon and low diversity libraries on Illumina instruments? An Illumina Field Applications Scientist discusses considerations for designing and performing amplicon sequencing.

    6 min


  • How Do I Optimize Amplicon Sequencing Data? Part 2

    An Illumina Field Applications Scientist discusses best practices for sequencing amplicons on Illumina instruments with high-quality results. We use Sequencing Analysis Viewer (SAV) to compare key metrics of amplicon sequencing runs to a standard PhiX run.

    7 min


  • Illumina Experiment Manager

    This course provides an introduction to Illumina Experiment Manager, and shows you the steps to create sample plates and sample sheets.

    25 min


  • Preventing Contamination

    Many next-generation sequencing applications use the Polymerase Chain Reaction, or PCR, for DNA amplification. This video presents best practices to minimize the potential for PCR contamination in your experiment.

    10 min


  • Nextera XT: Sample Preparation and Usage

    Recorded Webinar (August 2016) | Join Illumina Technical Support for a presentation and discussion on the Nextera XT sample preparation process. This session reviews the following topics: Nextera XT protocol, best practices for critical steps, and a comparison of the Nextera XT kit to the Nextera DNA kit, focusing on the applications recommended with each kit.  


  • Library QC and Troubleshooting with the BioAnalyzer

    Upcoming Webinar | Does my library look good to sequence? What’s that peak? This webinar will address how to use the Agilent BioAnalyzer to check library quality prior to sequencing, and troubleshoot sample preparation. This webinar is geared toward new and intermediate users, and will cover how to identify features of an ideal library trace, recognize potential issues, and prevent potential issues.

    Feb 25, 2020 PST