Reagents provided in the TruSeq Synthetic Long-Read DNA Library Prep Kit and TruSeq Synthetic Long-Read DNA Barcode Kit.
Designed for two applications: preparing DNA libraries for long-read assembly and phasing analysis from whole human genome sequencing data.
Leverages TruSeq and Nextera chemistries with the high accuracy of short sequencing reads to construct long synthetic fragments with high assembly accuracy or efficient phasing of whole human genome sequencing data.
Enables phasing of de novo mutations and the identification of co-inherited alleles in a population, providing greater insight into the human genome.
Long-read application—Generates synthetic long-read fragments that can improve the accuracy of genome construction by providing data on traditionally challenging regions, such as repetitive content. Enables more accurate, long contigs for de novo assembly, genome finishing, or metagenomics applications.
Phasing application—Designed for preparing human DNA libraries for phasing analysis. Combined with whole human genome sequencing variant data, this method assigns highly accurate shorter reads into long haplotype fragments for allele-specific analysis.