bcl2fastq Conversion Software

Latest Updates

bcl2fastq2 Conversion Software v2.19 Guide
bcl2fastq Conversion Software v1.8.4 User Guide

bcl2fastq Overview

bcl2fastq Conversion Software both demultiplexes data and converts BCL files generated by Illumina sequencing systems to standard FASTQ file formats for downstream analysis.

For Illumina sequencing systems running RTA version 1.18.54 and later, use bcl2fastq2 Conversion Software v2.17 or later.

For Illumina sequencing systems runnings RTA versions earlier than 1.18.54, use bcl2fastq Conversion Software v1.8.4.