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bcl2fastq2 Conversion Software v2.20

This download contains the software, release notes, and software guide for the bcl2fastq2 Conversion Software v2.20.0, which can be used to both demultiplex data and convert BCL files to FASTQ file formats for downstream analysis. This software requires a Linux operating system.

The bcl2fastq2 Conversion Software v2.20.0 can be used to convert BCL files from MiniSeq, MiSeq, NextSeq, HiSeq, and NovaSeq sequening systems. For conversion of data generated on Illumina sequencing systems using versions of RTA earlier than RTA 1.18.54, use bcl2fastq v1.8.4.

The software is available as an RPM (*.rpm) for installation on CentOS/RedHat Linux distributions. A tarball (*.tar.gz) of the source code is also available for compilation on other Linux distributions. Installation instructions are provided in the Software Guide.

bcl2fastq2 Conversion Software v2.19.1

This download contains the software, release notes, and software guide for the bcl2fastq2 Conversion Software v2.19.1, which can be used to both demultiplex data and convert BCL files to FASTQ file formats for downstream analysis. This software requires a Linux operating system.

The bcl2fastq2 Conversion Software v2.19.1 can be used to convert BCL files from MiniSeq, MiSeq, NextSeq, HiSeq, and NovaSeq sequening systems. For conversion of data generated on Illumina sequencing systems using versions of RTA earlier than RTA 1.18.54, use bcl2fastq v1.8.4.

The software is available as an RPM (*.rpm) for installation on CentOS/RedHat Linux distributions. A tarball (*.tar.gz) of the source code is also available for compilation on other Linux distributions. Installation instructions are provided in the Software Guide.

bcl2fastq Conversion Software v1.8.4

This download contains the software, release notes, and user guide for bcl2fastq Conversion Software v1.8.4, which can be used to demultiplex data and convert BCL files to FASTQ file formats for downstream analysis. This software requires a Linux operating system.

The bcl2fastq Conversion Software v1.8.4 software can be used to convert BCL files from MiSeq and HiSeq sequencing systems running RTA versions earlier than 1.8. The bcl2fastq Conversion Software v1.8.4 added the ability to mask multiple adapter sequences per read and configure stringency for the adapter masking feature. The software includes standard Illumina adapter sequences.