DRAGEN secondary analysis Product Files

To reconstruct the DRAGEN multigenome reference use all the files provided from same the reference build.

To customize the DRAGEN multigenome reference, use msVCF, and any of the other resource files.

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Collection of panel of normals files (PON) (combined.counts.txt.gz) for TruSight Hereditary Cancer Panel.

These are pre-constructed PON files. For optimal performance, it is recommended that you generate your own PON best matched to your lab's protocol.

Illumina DRAGEN CNV - Somatic TO (tumor-only) 

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Illumina DRAGEN Germline ASCN CNV

To be used in tumor-only workflows to identify candidate germline heterozygous sites used to estimate B-allele profile in tumor samples.

To be used in germline ASCN workflows to identify candidate sites used to estimate B-allele profile in germline samples.

Illumina DRAGEN scRNA, CRISPR, scATAC

Illumina DRAGEN Bulk RNA

Versions of GTF gene annotations downloaded from https://www.gencodegenes.org/ for use with the corresponding genome assembly: hg38, hg19, hs37d5, hs37d5-chr, hg38-mm39

Each  annotation contains the comprehensive gene annotation on the reference chromosomes only (excluding scaffolds, assembly patches and alternate loci).

Gene annotation input used for validation and benchmarking of the indicated version of DRAGEN software.

gencode_hs37d5_chr.v19.annotation.gtf tested for bulk RNA pipeline

gencode.hg38_v44.mm39_vM30.annotation.gtf.gz tested for  scRNA pipeline only

gencode.v44.annotation.gtf.gz,  gencode.v19.annotation.gtf  tested for all pipelines.

You may use a different annotation on an "as needed" basis at your discretion (eg, different assemblies or species).

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To be used only to build a custom multigenome reference for DRAGEN v.4.0, DRAGEN v.4.1 and DRAGEN v4.2.

Note: The DRAGEN multigenome reference cannot be reconstructed with these resource files.

ORA Compression Reference All species V2.0