Downloads

Downloads

Description
 
File Info
Date Posted
Enrichment Analysis Workflow for Nextera Rapid Capture data

This demo dataset has been provided, by Illumina, so that users can validate the installation of the Enrichment workflow within the HiSeq Analysis Software (HAS). The data folder (NexteraRapidCapture_DemoData) contains a Run Folder and a README.txt. Briefly, the Run Folder contains 10% of tiles from one lane of reads belonging to the Coriell Sample: GM10861 (http://ccr.coriell.org/Sections/Search/Sample_Detail.aspx?Ref=GM10861) enriched using the Nextera Rapid Capture Exome Kit and sequenced as a 12plx run. Details on running HAS, expected run time, input and output files can be found in the README.txt. The tar.gz folder can be unzipped using the following command: $tar -zxvf NexteraRapidCapture_DemoData

Nextera Rapid Capture Exome Targeted Regions Manifest v1.2 ReadMe

This document describes the changes introduced in the Nextera Rapid Capture Exome v1.2 manifest files.

HiSeq Analysis Software hg19 Reference Genome

This download contains the human reference genome hg19 from UCSC for the HiSeq Analysis Software (tar).

HiSeq Analysis Software v0.9

This download contains the source files, scripts, and documentation necessary for installing and running the HiSeq Analysis Software, Version 0.9, on a Linux operating system. The changes are recorded in the Release Notes available in the documentation.

Nextera Rapid Capture Exome Unique Intervals File

"This download contains the Nextera Rapid Capture Exome target region intervals that do not overlap the Nextera Rapid Capture Expanded Exome target regions. There are a total of 4.1 MB that are unique to the Exome product. 336,295 intervals across 205,598 Exome targeted regions. This was determined by base overlap only. For example, comparing an Exome product region at Chr 1 position 300 – 500 with an Expanded Exome region at Chr 1 position 305 – 495 results in a 191 bp overlap and two 5 bp ""unique regions"" for the Exome product."

Nextera Rapid Capture Expanded Exome Unique Intervals File

"This download contains the Nextera Rapid Capture Expanded Exome target region intervals that do not overlap the Nextera Rapid Capture Exome target regions. There are a total of 29 MB that are unique to the Expanded Exome product. 50,257 intervals across 47,273 Expanded Exome targeted regions. This was determined by base overlap only. For example, comparing an Exome product region at Chr 1 position 300 – 500 with an Expanded Exome region at Chr 1 position 305 – 495 results in a 191 bp overlap and two 5 bp ""unique regions"" for the Exome product."

Nextera Rapid Capture Exome vs. Expanded Exome Overlap File

"This download contains the Nextera Rapid Capture Exome targeted region intervals that overlap the Nextera Rapid Capture Expanded Exome targeted regions. The total overlap is 33.0 MB between the two products. 189,522 overlapping intervals across 188,621 Exome targeted regions. This was determined by base overlap only. For example, comparing an Exome product region at Chr 1 position 300 – 500 with an Expanded Exome region at Chr 1 position 305 – 495 results in a 191 bp overlap and two 5 bp ""unique regions"" for the Exome product."

Nextera Rapid Capture Exome Product Files

This download contains the Nextera Rapid Capture Exome targeted regions manifest files (.txt and .bed), and the exome probe manifest. The targeted regions manifest files list the 212,158 targeted exonic regions with start and stop chromosome locations in GRCh37/hg19. The exome probe manifest file lists the 412,006 enrichment probes with chromosome location in GRCh37/hg19.

The Nextera Rapid Capture Exome Targeted Regions Manifest and the Nextera Rapid Capture Exome Probe Manifest files are in .txt format. This format is required by the HiSeq Analysis Software.

Nextera Rapid Capture Expanded Exome Product Files

This download contains the Nextera Rapid Capture Expanded Exome targeted regions manifest files (.txt and .bed), and the exome probe manifest. The targeted regions manifest files list the 201,071 targeted exonic regions with start and stop chromosome locations in GRCh37/hg19. The expanded exome probe manifest file lists the 347,517 enrichment probes with chromosome location in GRCh37/hg19.

The Nextera Rapid Capture Expanded Exome Targeted Regions Manifest and the Nextera Rapid Capture Expanded Exome Probe Manifest files are in .txt format. This format is required by the HiSeq Analysis Software.

 

Nextera Rapid Capture Exome v1.2 Product Files

This download contains the Nextera Rapid Capture Exome v1.2 release notes, targeted regions manifest files (.txt and .bed), and the exome probe manifest. The readme document describes the Nextera Rapid Capture Exome v1.2 manifest files. The targeted regions manifest files list the 214,126 targeted exonic regions with start and stop chromosome locations in GRCh37/hg19.

The Nextera Rapid Capture Exome v1.2 Targeted Regions Manifest and the Nextera Rapid Capture Exome Probe Manifest files are in .txt format. This format is required by the HiSeq Analysis Software.