Prep Libraries
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1.
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In the Libraries page, select the Library Prep Kit drop-down arrow, and select a kit. |
Note
If your kit is not listed but is compatible with your sequencing system, set up a custom library prep kit or select a kit that uses the same index adapter set. For example, select Nextera Rapid Capture for TruSight Cardio.
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2.
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In the Plate ID field, enter a unique plate ID. |
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3.
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[Optional] In the Notes field, enter any notes. For Nextera Rapid Capture libraries, specify that the plate contains multiple enrichments. |
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4.
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[Optional] Assign the sample to a different project. |
- Select the checkbox of each library to assign to a project.
- Select Set Project.
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In the Select Project dialog, select or create a project to assign libraries to, and then select Select.
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5.
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Select Auto Prep to automatically assign each library to a well and index location. For Nextera Rapid Capture libraries, samples belonging to the same enrichment must be in the same row. |
Each plate has a maximum layout of 12 indexes by 8 indexes. If you require more wells or indexes, create multiple plates.
Note
Biological samples are assigned an index even if you are not performing indexed sequencing. During run setup, you can specify no indexing.
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6.
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[Optional] To change indexes, select an index on the plate and select a new index from the drop-down list. |
NOTE
This option is not available for fixed layout plates.
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7.
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[Optional] Move libraries to a different well as follows. |
- Select the checkbox of the library to move.
- Drag the selected library from the Libraries area to the appropriate well.
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8.
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Select Export to save your library prep settings in a .csv file. |
This file serves as a reference when preparing samples in the lab.
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9.
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[Optional] Select additional plates as follows. |
- SelectSave and Continue Later to return to Libraries.
- Select the checkbox of each library you want to use.
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10.
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Select Pool Libraries. |