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View Indexing QC Results

Available only for indexed runs, the Indexing QC page provides indexing QC results for the run. View unexpected results for a sample, or confirm that all indexed samples are properly represented.

Select the Indexing QC tab from a run page to view indexing QC for the run.

The first table summarizes the indexing performance for the selected lane. Data on the frequency of an index is provided in the second table and the graph.

First Table - Index Performance Summary

Column

Description

Total Reads

Total number of reads.

PF Reads

Total number of clusters passing filter.

% Reads Identified (PF)

Percentage of clusters passing filter that are assigned to an index.

% Reads Undetermined

Percentage of clusters passing filter that could not be assigned to an index.

CV

Coefficient of variation for the number of counts across all indexes.

Min

Lowest representation for any index.

Max

Highest representation for any index.

Second Table - Index Frequency

Column

Description

Index Number

A unique number BaseSpace Sequence Hub assigns to each index for display purposes.

Sample ID

The sample ID assigned to an index in the sample sheet.

Library Name

The library assigned to an index in the sample sheet.

Index 1 (i7)

The sequence for the first index read.

Index 2 (i5)

The sequence for the second index read.

% Reads Identified (PF)

Percentage of clusters passing filter that are assigned to the index.