Before preparing libraries, record information about your samples using an option available for your sequencing system:
AmpliSeq for Illumina Immune Repertoire Plus, TCR beta Panel is a highly multiplexed targeted resequencing panel to measure T cell diversity and clonal expansion by sequencing T-cell receptor (TCR) beta chain rearrangements.
|Panel||Number of Pools||Concentration|
|AmpliSeq for Illumina Immune Repertoire Panel||1||5X|
Completing the AmpliSeq for Illumina Immune Repertoire protocol requires the panel and the following library prep kit and index kit. The AmpliSeq for Illumina Library PLUS Kit is available in 24-, 96-, and 384-reaction configurations.
For each RNA sample, AmpliSeq for Illumina Immune Repertoire generates one RNA library. A sample is a specimen of nucleic acid from one source, while a library is nucleic acids from a sample after preparation. A library has the ends required for sequencing.
Preparing the appropriate number of samples requires the following number and combination of kits.
Number of Samples
|96 Samples||384 Samples|
|Number of Libraries||24 Libraries||96 Libraries||384 Libraries|
|AmpliSeq for Illumina Immune Repertoire Panel||1||4||16|
|AmpliSeq Library PLUS For Illumina||1 24-reaction kit||1 96-reaction kit||1 384-reaction kit|
|AmpliSeq CD Set A (96 reaction, 96 indexes)||1||1||4|
Sequence AmpliSeq for Illumina Immune Repertoire Panel libraries using the following systems and reagent kits.
|MiniSeq System||MiniSeq Mid Output Reagent Kit|
|MiniSeq High Output Reagent Kit|
|MiSeq System||MiSeq Reagent Kit v3|
|NextSeq System||NextSeq Mid Output v2 Kit|
|NextSeq High Output v2 Kit|