AmpliSeq for Illumina Myeloid Panel Compatible Products

Sample Information

Before preparing libraries, record information about your samples using an option available for your sequencing system:

Library Prep

Panel Description

The AmpliSeq for Illumina Myeloid Panel allows concurrent analysis of both DNA and RNA from blood and bone marrow samples to study biomarkers associated with hematologic malignancies. The panel has separate pools for DNA and RNA, so they can be be processed separately or together.

Component Number of Pools Concentration Number of Amplicons Average Amplicon Length (bp) Average Library Length (bp)
DNA 2 5X 526 230 370
RNA 1 5X 700 100 240
Library Prep and Index Kits

Completing the AmpliSeq for Illumina Myeloid Panel protocol requires the panel and the following library prep, cDNA synthesis, and index kits. The AmpliSeq for Illumina Library PLUS Kit is available in 24-, 96-, and 384-reaction configurations.

Number of Samples

The AmpliSeq for Illumina Myeloid Panel protocol generates one DNA and one RNA library per sample. Samples can be DNA, RNA, or paired. Samples for this protocol are paired. A sample is a specimen of nucleic acid from one source, while a library is nucleic acids from a sample after preparation. A library is a unique DNA or RNA preparation that has the ends required for sequencing.

Preparing the appropriate number of samples requires the following number and combination of kits.

Number of Samples 24 Samples 96 Samples 384 Samples
Number of Libraries 48 Libraries
(24 DNA, 24 RNA)
192 Libraries
(96 DNA, 96 RNA)
768 Libraries
(384 DNA, 384 RNA)
AmpliSeq for Illumina Myeloid Panel 1 4 16
AmpliSeq Library PLUS For Illumina 2 24-reaction kits 2 96-reaction kits 2 384-reaction kits
AmpliSeq CD Set A (96 reaction, 96 indexes) 1 2 8
cDNA Synthesis 1 1 4

Sequencing Guidelines

Sequence AmpliSeq for Illumina Myeloid Panel libraries using the following systems and reagent kits.

System Reagent Kit Max # DNA
Samples Only per Run
Max # RNA
Samples Only per Run
Max # Combined*
Samples per Run
DNA:RNA Pooling
Volume Ratio**
Run Time
MiniSeq System MiniSeq Mid Output Reagent Kit 4 32 3 8:1 17 hours
  MiniSeq High Output Reagent Kit 12 96 11 8:1 24 hours
MiSeq System MiSeq Reagent Kit v2 7 60 6 8:1 24 hours
  MiSeq Reagent Kit v3 12 96 11 8:1 32 hours
NextSeq System NextSeq Mid Output v2 Kit 65 96 48 8:1 26 hours
  NextSeq High Output v2 Kit 96 96 48 8:1 29 hours

* Combined means paired DNA and RNA from the same sample that results in two libraries, one generated from each nucleic acid type and indexed separately.

** Combined DNA to RNA pooling ratio based on read coverage recommendation.