Contents & Storage

Contents & Storage

The Nextera DNA Library Prep Kit is provided with sufficient reagents to prepare up to 24 or 96 samples and shipped on dry ice unless specified otherwise below. Each kit has a corresponding Index Kit that contains 96 or 24 indices. Please note that a Nextera Index kit (FC-121-1011 or FC-121-1012) is required to complete the protocol, regardless of the sample pooling level used for sequencing.

Nextera DNA Library Preparation Kit (96 Samples)

Reagent

Storage

Description

TD

-15° to -25°C

Tagment DNA Buffer

TDE1

-15° to -25°C

Tagment DNA Enzyme

NPM

-15° to -25°C

Nextera PCR Master Mix

PPC

-15° to -25°C

PCR Primer Cocktail

RSB

-15° to -25°C

Resuspension Buffer

Nextera Index Kit (96 Indices, 384 Samples)

Reagent

Storage

Description

Index Primers

-15° to -25°C

N502 to N508, and N517

Index Primers

-15° to -25°C

N701 to N712

Nextera DNA Library Preparation Kit (24 Samples)

Reagent

Storage

Description

TD

-15° to -25°C

Tagment DNA Buffer

TDE1

-15° to -25°C

Tagment DNA Enzyme

NPM

-15° to -25°C

Nextera PCR Master Mix

PPC

-15° to -25°C

PCR Primer Cocktail

RSB

-15° to -25°C

Resuspension Buffer

Nextera Index Kit (24 Indices, 96 Samples)

Reagent

Storage

Description

Index Primers

-15° to -25°C

N502 to N504, and N517

Index Primers

-15° to -25°C

N701 to N706

For safety information, see the Safety Data Sheet for the Nextera DNA Library Preparation Index Adapter and the Safety Data Sheet for the Nextera DNA Library Preparation Kit.