Before preparing libraries, record information about your samples using an option available for your sequencing system.
Completing the TruSeq DNA PCR-Free protocol requires the library prep kit and a compatible index kit.
After library preparation, denature and dilute libraries to the appropriate loading concentration using the instructions for standard normalized libraries.
FASTQ, VCF, and BAM files are available for the following HiSeq 2500 data sets. Viewing the data sets requires signing in to BaseSpace Sequence Hub.