The MiSeq Reporter requires the following primary analysis files to begin secondary analysis. The software automatically accesses these files in the run folder.
|RTAComplete.txt||Marker file that indicates that RTA processing is complete.|
|SampleSheet.csv||Provided at the beginning of a sequencing run. Contains analysis workflow and important run information.|
|RunInfo.xml||Identifies high-level run information.|
|*.bcl files||Files contain base calling information.|
|*.filter files||Files contain filter results for each tile.|
|*.locs files||Files contain the locations of all clusters on a tile.|
miRbase for human
dbSNP for human
refGene for human
E. coli strain DH10b
human (Homo sapiens) build hg19
You can upload and use your own reference in FASTA format. Reference FASTA files should be stored in a single folder and have a *.fa or *.fasta extension.