DNA Library QC Metrics for Copy Number Variant Calling
Metric |
Description |
Recommended Threshold |
---|---|---|
PCT_CHIMERIC_READS (%) |
Percentage of unique reads that are part of a chimeric alignment (for example, half of the read maps to one location and the other half maps to a nonsequential location) out of the total number of mapped unique reads. |
≤ 3.5 |
COVERAGE_MAD |
Median of absolute deviations from the median of the normalized count of each CNV target region. |
≤ 0.2 |
MEDIAN_BIN_COUNT_CNV_TARGET |
Median of the raw read counts (normalized by region size) of each CNV target region. |
≥ 1 |