RNA Expanded Metrics
|
Metric |
Description |
Recommended Threshold |
|---|---|---|
|
PCT_CHIMERIC_READS (%) |
Percentage of unique reads that are part of a chimeric alignment (for example, half of the read maps to one location and the other half maps to a nonsequential location) out of the total number of mapped unique reads. |
Not applicable |
|
TOTAL_PF_READS (Count) |
Total number of reads passing filter. |
Not applicable |
|
PCT_ON_TARGET_READS |
Percentage of reads that cross any part of the target region out of the total number of reads. A read that partially maps to a target region is counted as on target. |
Not applicable |
|
SCALED_MEDIAN_GENE_COVERAGE |
The median of median gene coverage scaled by gene length. Median coverage is calculated for each gene, and then scaled by the respective gene’s length, and finally divided by the total length of all targeted genes. |
Not applicable |