RNA Library QC Metrics
|
Metric |
Description |
Recommended Threshold |
|---|---|---|
|
MEDIAN_INSERT_SIZE (bp) |
Median of fragment sizes calculated from read alignments. |
≥ 80 |
|
MEDIAN_CV_GENE_500X |
The median of the CV value of target region coverage across all genes with coverage above 500X. |
≤ 0.93 |
|
TOTAL_ON_TARGET_READS (Count) |
Total number of reads that map to the target regions. |
≥ 9,000,000 |