Requirements & Compatibility

Requirements & Compatibility

Version Compatibility of Run Components

Kit Version

Recipe Version

Software Version

HiSeq 3000/4000 Cluster Kit

Version 1.0 recipes

cBot v3.0.46, or later (SR kit)
cBot v2.0.34, or later (PE kit)

HiSeq X Ten Reagent Kit v2.5

Version 2.0 recipes

cBot v2.0.29, or later

HiSeq X Five Reagent Kit v2.5 Version 2.0 recipes cBot v2.0.29, or later
HiSeq Cluster Kit v4 Version 9.0 recipes cBot v2.0.16, or later
HiSeq Rapid Duo cBot Sample Loading Kit Version R recipes cBot v1.5, or later
TruSeq Rapid Duo cBot Sample Loading Kit Version R recipes cBot v1.5, or later
TruSeq Cluster Kit v3 - HS Version 8.0 recipes cBot v1.4, or later
TruSeq Cluster Kit v2 - GA Version 7.0 recipes cBot v1.3, or later

Read 1 Sequencing Primer for Nextera Libraries

HP10 is required for sequencing Nextera libraries on a TruSeq v3 or TruSeq v2 flow cell. HP10 is provided in the TruSeq Dual Index Sequencing Primer Box.

Kit Name

Recipe Version

Software Version

TruSeq Dual Index Sequencing Primer Box, Single Read
Catalog # FC-121-1003

Version 8.0 recipes (HiSeq)

Version 7.0 recipes (GAIIx)

cBot v1.4.36 or later

TruSeq Dual Index Sequencing Primer Box, Paired End
Catalog # PE-121-1003

Version 8.0 recipes (HiSeq)

Version 7.0 recipes (GAIIx)

cBot v1.4.36 or later

Cluster Kits for the HiSeq X

Kit Name

Catalog #

HiSeq X Ten Reagent Kit v2.5

FC-501-2501

HiSeq X Ten Reagent Kit v2.5 (10 pack)

FC-501-2521

HiSeq X Five Reagent Kit v2.5

FC-502-2501

HiSeq X Five Reagent Kit v2.5 (10 pack)

FC-502-2102

Cluster Kits for the HiSeq

Kit Name

Catalog #

HiSeq 3000/4000 SR Cluster Kit

GD-410-1001

HiSeq 3000/4000 PE Cluster Kit

PE-410-1001

HiSeq SR Cluster Kit v4

GD-401-4001

HiSeq PE Cluster Kit v4

PE-401-4001

TruSeq SR Cluster Kit v3 - HS

GD-401-3001

TruSeq PE Cluster Kit v3 - HS

PE-401-3001

TruSeq Rapid Duo cBot Sample Loading Kit

CT-403-2001

TruSeq Rapid Duo cBot Sample Loading Kit

CT-402-4001

Cluster Kits for the Genome Analyzer

Kit Name

Catalog #

TruSeq SR Cluster Kit v2 - GA (cBot)

GD-300-2001

TruSeq PE Cluster Kit v2 - GA (cBot)

PE-300-2001

Rehybridization Kits

Kit Name

Catalog #

HiSeq X cBot Multi-Primer Rehybridization Kit v2

GD-305-2001

HiSeq 3000/4000 cBot Multi-Primer Rehybridization Kit

GD-310-1001

TruSeq v2 cBot Multi-Primer Rehybridization Kit

GD-304-2001