Requirements & Compatibility

SBS Kits for the HiSeq 2500

Cluster Kits for the HiSeq 2500

Sequencing Primers for Dual-Indexed Libraries

Sequencing primers provided in the TruSeq Dual Index Sequencing Primer Box are required for sequencing the following libraries on the HiSeq 2500:

  • Any Nextera libraries with any number of indices for single-read or paired-end runs. Use the single-read kit with single-read flow cells, and the paired-end kit with paired-end flow cells.
  • TruSeq HT libraries when performing a dual-indexed run on a single-read flow cell.

For instructions, see the HiSeq 2500 User Guide.

Kit and Software Versions

High Output

Cluster Kit Version SBS Kit Version Software Version
HiSeq Cluster Kit v4 with Flow Cell v4 HiSeq SBS Kit v4 HCS v2.2 or later
TruSeq Cluster Kit v3 with Flow Cell v3 TruSeq SBS Kit v3 HCS v2.0 or later

Rapid Run

Cluster Kit Version SBS Kit Version Software Version
HiSeq Rapid PE Cluster Kit v2 with Rapid Flow Cell v2
HiSeq Rapid SR Cluster Kit v2 with Rapid Flow Cell v2
HiSeq Rapid Duo cBot Sample Loading Kit
HiSeq Rapid SBS Kit v2 HCS v2.2 or later
TruSeq Rapid PE Cluster Kit with Rapid Flow Cell
TruSeq Rapid SR Cluster Kit with Rapid Flow Cell
TruSeq Rapid Duo cBot Sample Loading Kit
TruSeq Rapid SBS Kit - HS HCS v2.0 or later

Additional Kits

Kit Software Version
TruSeq Dual Index Sequencing Primer Box HCS v2.0 / RTA v1.17, or later