Documentation & Literature

The safety and compliance guide describes safety considerations and lists product compliance markings for the HiSeq 2500 system.

This guide provides an overview of instrument components and instructions for operating and maintaining the HiSeq 2500. Workflows include HiSeq v4, TruSeq v3, and Rapid Run.

This guide provides lab specifications and requirements for preparing the lab for sequencing on the HiSeq 2500, HiSeq 1500, or HiSeq 2000 system.

This guide contains instructions for denaturing and diluting libraries after library preparation and before sequencing on a HiSeq system. This guide also includes instructions for preparing a PhiX control.

This guide provides an overview of the indexed sequencing process for single-indexing and dual-indexing workflows on the iSeq, NovaSeq, MiniSeq, NextSeq, MiSeq, and HiSeq sequencing systems.

This guide contains instructions for using custom primers for a sequencing run on the HiSeq 2500, HiSeq 1500, HiSeq 2000, HiSeq 3000, or HiSeq 4000 system.

This guide contains instructions for performing primer rehybridization on the HiSeq using the HiSeq Multi-Primer Rehyb Kit v4. Instructions for a TruSeq v3 Read 2 primer rehybridization are also included.

This guide contains instructions for performing primer rehybridization on the HiSeq using the HiSeq Rapid Rehybridization Kit or the TruSeq Rapid Rehybridization Kit.

This guide contains instructions for sequencing dual-indexed libraries on the HiSeq.