HiSeq 2500 Documentation

  • Supporting Documents

    SIZE

    DATE


  • Primer Rehybridization on a HiSeq High Output Flow Cell

    Primer rehybridization instructions using HiSeq v4 or TruSeq v3 chemistry.

    Download

    < 1 MB

    Dec 17, 2014


  • Primer Rehybridization on a HiSeq Rapid Flow Cell

    Instructions for performing primer rehybridization on the HiSeq using the HiSeq Rapid Rehybridization Kit or the TruSeq Rapid Rehybridization Kit.

    Download

    < 1 MB

    Dec 17, 2014


  • Read 1 Primer Rehyb on a TruSeq v3 Flow Cell

    Instructions for using the TruSeq v2 cBot Multi-Primer Re-Hybridization Kit for primer rehybridization on a TruSeq v3 Flow Cell.

    Download

    < 1 MB

    Aug 28, 2018


  • Cluster Optimization Overview

    Strategies for optimizing cluster density and preventing and diagnosing clustering issues on Illumina flow cells.

    View Online Help

    Apr 26, 2021


  • Indexed Sequencing Overview

    The sequencing process for single- and dual-indexed runs on Illumina instruments.

    View Online Help

    Aug 13, 2024


  • Security and Networking

    Guidelines for managing the security of the control computer on your sequencing system. Use these guidelines to configure your system and ensure a more secure operating environment.

    It is strongly recommended that you review this documentation to keep your instrument secure.

    View Online Help

    Jan 12, 2024


Product Literature

TYPE

SIZE

DATE

HiSeq Applications Brochure
Download
Brochure 4 MB
Illumina Sequencing Portfolio Brochure
Download
Brochure 9 MB Mar 5, 2015
Reducing Whole-Genome Data Storage Footprint
Download
White paper < 1 MB Oct 10, 2012
Data Analysis: GWAS Processing
Download
Technical note < 1 MB Aug 29, 2014
Data Correlation Details: Enrichment Analysis
Download
Technical note < 1 MB Aug 29, 2014
DNA-Seq Data Processing
Download
Technical note < 1 MB Aug 29, 2014
Low-Diversity Sequencing on the Illumina HiSeq Platforms
Download
Technical note < 1 MB Aug 20, 2014
Meta-Analysis Framework
Download
Technical note < 1 MB Aug 29, 2014
A Patient-Centric SNV-CNV Pipeline
Download
Technical note < 1 MB Aug 29, 2014
RNA-Seq Data Processing
Download
Technical note < 1 MB Aug 29, 2014