iSeq 100 Documentation

System Documents

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iSeq 100 Sequencing System Setup Poster

Instructions on installing and setting up the iSeq 100 Sequencing System.

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iSeq 100 Sequencing System Setup Poster (1000000035963 v05)

< 1 MB

Aug 3, 2020

iSeq 100 Sequencing System Setup Poster Translated into Arabic

2 MB

Aug 26, 2020

iSeq 100 Sequencing System Setup Poster Translated into Armenian

2 MB

Aug 26, 2020

iSeq 100 Sequencing System Setup Poster Translated into Brazilian Portuguese

2 MB

Aug 26, 2020

iSeq 100 Sequencing System Setup Poster Translated into Bulgarian

2 MB

Aug 26, 2020

iSeq 100 Sequencing System Setup Poster Translated into Czech

2 MB

Aug 26, 2020

iSeq 100 Sequencing System Setup Poster Translated into Danish

2 MB

Aug 26, 2020

iSeq 100 Sequencing System Setup Poster Translated into Dutch

2 MB

Aug 26, 2020

iSeq 100 Sequencing System Setup Poster Translated into Estonian

2 MB

Aug 26, 2020

iSeq 100 Sequencing System Setup Poster Translated into Finnish

2 MB

Aug 26, 2020

iSeq 100 Sequencing System Setup Poster Translated into French

2 MB

Aug 26, 2020

iSeq 100 Sequencing System Setup Poster Translated into German

2 MB

Aug 26, 2020

iSeq 100 Sequencing System Setup Poster Translated into Greek

2 MB

Aug 26, 2020

iSeq 100 Sequencing System Setup Poster Translated into Hebrew

2 MB

Aug 26, 2020

iSeq 100 Sequencing System Setup Poster Translated into Hungarian

2 MB

Aug 26, 2020

iSeq 100 Sequencing System Setup Poster Translated into Italian

2 MB

Aug 26, 2020

iSeq 100 Sequencing System Setup Poster Translated into Japanese

1 MB

Aug 26, 2020

iSeq 100 Sequencing System Setup Poster Translated into Korean

1 MB

Sep 8, 2020

iSeq 100 Sequencing System Setup Poster Translated into Latvian

2 MB

Aug 26, 2020

iSeq 100 Sequencing System Setup Poster Translated into Lithuanian

2 MB

Aug 26, 2020

iSeq 100 Sequencing System Setup Poster Translated into Maltese

2 MB

Aug 26, 2020

iSeq 100 Sequencing System Setup Poster Translated into Norwegian

2 MB

Aug 26, 2020

iSeq 100 Sequencing System Setup Poster Translated into Portuguese

2 MB

Aug 26, 2020

iSeq 100 Sequencing System Setup Poster Translated into Russian

2 MB

Aug 26, 2020

iSeq 100 Sequencing System Setup Poster Translated into Simplified Chinese

2 MB

Aug 26, 2020

iSeq 100 Sequencing System Setup Poster Translated into Slovak

2 MB

Aug 26, 2020

iSeq 100 Sequencing System Setup Poster Translated into Slovenian

2 MB

Aug 26, 2020

iSeq 100 Sequencing System Setup Poster Translated into Spanish

2 MB

Aug 26, 2020

iSeq 100 Sequencing System Setup Poster Translated into Swedish

2 MB

Aug 26, 2020

iSeq 100 Sequencing System Setup Poster Translated into Traditional Chinese

2 MB

Aug 26, 2020

iSeq 100 Sequencing System Setup Poster Translated into Turkish

2 MB

Aug 26, 2020

iSeq 100 Sequencing System Setup Poster Translated into Ukrainian

2 MB

Aug 26, 2020

iSeq 100 Sequencing System Setup Poster Translated into Vietnamese

2 MB

Aug 26, 2020

RFID Reader Compliance Guide

Safety considerations, compliance markings, and regulatory statements for the RFID reader module, an internal instrument component.

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RFID Reader Module Compliance Guide (1000000002699 v05)

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Apr 13, 2020

RFID Reader Module Compliance Guide in Arabic

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May 26, 2020

RFID Reader Module Compliance Guide in Armenian

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May 26, 2020

RFID Reader Module Compliance Guide in Brazilian Portuguese

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May 26, 2020

RFID Reader Module Compliance Guide in Bulgarian

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Simplified Chinese

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Traditional Chinese

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May 26, 2020

RFID Reader Module Compliance Guide in Czech

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May 26, 2020

RFID Reader Module Compliance Guide in Danish

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May 26, 2020

RFID Reader Module Compliance Guide in Dutch

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May 26, 2020

RFID Reader Module Compliance Guide in Estonian

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Finnish

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in French

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May 26, 2020

RFID Reader Module Compliance Guide in German

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Greek

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Hebrew

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Hungarian

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Italian

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Japanese

< 1 MB

Oct 15, 2020

RFID Reader Module Compliance Guide in Korean

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Nov 3, 2020

RFID Reader Module Compliance Guide in Latvian

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May 26, 2020

RFID Reader Module Compliance Guide in Lithuanian

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May 26, 2020

RFID Reader Module Compliance Guide in Maltese

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Norwegian

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Polish

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Portuguese

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Russian

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Slovak

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Slovenian

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Spanish

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Swedish

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Turkish

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May 26, 2020

RFID Reader Module Compliance Guide in Ukrainian

< 1 MB

May 26, 2020

RFID Reader Module Compliance Guide in Vietnamese

< 1 MB

May 26, 2020

iSeq 100 Product Documentation

System overview and instructions for operating and maintaining the iSeq 100 Sequencing System.

View Online Help

iSeq 100 Sequencing System (200015511 v02)

Mar 18, 2024

iSeq 100 Sequencing System Translated into Brazilian Portuguese

Oct 17, 2023

iSeq 100 Sequencing System Translated into Korean

Aug 29, 2023

iSeq 100 Sequencing System Translated into Japanese

Sep 8, 2023

iSeq 100 Sequencing System Translated into Spanish

Oct 17, 2023

Supporting Documents

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Cluster Optimization Overview

Strategies for optimizing cluster density and preventing and diagnosing clustering issues on Illumina flow cells.
View Online Help

Apr 26, 2021

Indexed Sequencing Overview

The sequencing process for single- and dual-indexed runs on Illumina instruments.

View Online Help

Aug 13, 2024

Security and Networking

Guidelines for managing the security of the control computer on your sequencing system. Use these guidelines to configure your system and ensure a more secure operating environment.

It is strongly recommended that you review this documentation to keep your instrument secure.

This site is updated frequently. Make sure to visit this site often for the latest security recommendations.

View Online Help

Qualification Documents

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iSeq 100 Sequencing System Installation Qualification and Operational Qualification

Protocol to verify correct installation of the iSeq 100 Sequencing System.

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iSeq 100 Sequencing System Installation Qualification and Operational Qualification (1000000051068 v00)

< 1 MB

May 30, 2018

iSeq 100 Sequencing System Installation Qualification and Operational Qualification in French

< 1 MB

Oct 8, 2018

iSeq 100 Sequencing System Installation Qualification and Operational Qualification in Italian

< 1 MB

Oct 8, 2018

iSeq 100 Sequencing System Installation Qualification and Operational Qualification in German

< 1 MB

Oct 8, 2018

iSeq 100 Sequencing System Installation Qualification and Operational Qualification in Spanish

< 1 MB

Oct 8, 2018

iSeq 100 Sequencing System Performance Qualification

Protocol to verify correct performance of the iSeq 100 Sequencing System.

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iSeq 100 Sequencing System Performance Qualification (1000000052859 v00)

< 1 MB

May 30, 2018

iSeq 100 Sequencing System Performance Qualification in French

< 1 MB

Oct 8, 2018

iSeq 100 Sequencing System Performance Qualification in Italian

< 1 MB

Oct 8, 2018

iSeq 100 Sequencing System Performance Qualification in German

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Oct 8, 2018

iSeq 100 Sequencing System Performance Qualification in Spanish

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Oct 8, 2018

Product Literature	TYPE	SIZE	DATE
iSeq 100 System Brochure Download	Brochure	< 1 MB	Feb 9, 2018
16S Metagenomics Sequencing on the iSeq 100 System Download	Application note	4 MB	Aug 13, 2018
AmpliSeq for Illumina Oncology Panels on the iSeq 100 System Download	Application note	< 1 MB	Feb 27, 2018
Microbial Whole-Genome Sequencing App Note Download	Application note	5 MB	Jan 5, 2018
Sequencing Library QC on the iSeq System Download	Application note	< 1 MB	Nov 2, 2018
Illumina CMOS Chip and One-Channel SBS Chemistry Download	technology_spotlights	< 1 MB	Jan 16, 2014
Grant Assistance Document Download	Product information sheet	< 1 MB	Jan 8, 2018
Illumina Proactive Service connectivity guide - iSeq 100 Download	Brochure	< 1 MB	Jun 18, 2019
QC and rebalancing of single-cell gene expression libraries using the iSeq™ 100 System Download	Application note	2 MB	Jan 29, 2020
Pan-Viral Sequencing on Illumina Benchtop Systems Application Note Download	Application note
Illumina Sequencing Platforms Brochure Download	Brochure	1 MB	Oct 9, 2024
Illumina Proactive on iSeq 100 System Download	Brochure	< 1 MB
iSeq 100 System specification sheet Download	Data sheet	12 MB	Apr 29, 2025
Data Security with Illumina Proactive Download	Technical note	< 1 MB	Oct 4, 2022
Accurate surveillance of healthcare-acquired infections Download	Technical note	< 1 MB	Dec 13, 2021
Microbial whole-genome sequencing with the iSeq 100 System Download	Application note	< 1 MB

For every lab, everywhere

Illumina Single Cell 3' RNA Prep

NGS Workflow Finder

DRAGEN secondary analysis v4.4 now available

Illumina Proactive Instrument Performance Service

Do more, faster than ever

Next-generation sequencing for beginners

Next-generation sequencing for beginners

Next-generation sequencing for beginners

Next-generation sequencing for beginners

Next-generation sequencing for beginners

Next-generation sequencing for beginners

Next-generation sequencing for beginners

Illumina innovation roadmap

Illumina innovation roadmap

Illumina innovation roadmap

Illumina innovation roadmap

Illumina innovation roadmap

Illumina innovation roadmap

Illumina innovation roadmap

For every lab, everywhere

For every lab, everywhere

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iSeq 100 Documentation

iSeq 100 System

Documentation

System Documents

SIZE

DATE

iSeq 100 Sequencing System Setup Poster

RFID Reader Compliance Guide

iSeq 100 Product Documentation

Supporting Documents

SIZE

DATE

Cluster Optimization Overview

Indexed Sequencing Overview

Security and Networking

Qualification Documents

SIZE