iSeq 100 System Training

  • Introductory Topics


  • Sequencing: Illumina Technology

    This course provides a general overview of the Illumina sequencing workflow from extraction of nucleic acids to the completion of a sequencing run.

    Languages: French, Italian, German, SpanishChinese (Simplified), French,

    15 min

  • Sequencing: Fundamentals

    After completing this course you will be able to describe DNA, RNA and the central dogma; discuss traditional sequencing methodologies; and compare traditional sequencing to sequencing by synthesis.

    Languages: French, Italian, German, SpanishChinese (Simplified)

    15 min

  • Sequencing: Introduction to Sequencing By Synthesis (SBS)

    Recorded Webinar (June 2020) | Illumina next-generation sequencing technology allows for massive parallel sequencing. Illumina Technical Support invites you to join us for a presentation and discussion on sample preparation, cluster generation and sequencing by synthesis chemistry. This webinar is targeted to those new to next-generation sequencing and will discuss the following topics: Illumina library construction, cluster generation methods by platform, sequencing by synthesis, and multiplexing and primary analysis.

  • iSeq 100: Introduction

    Recorded Webinar (February 2020) | Illumina Technical Support invites you to join us for a discussion of the new iSeq 100 system. This webinar is targeted to new users with a basic understanding of the Illumina sequencing workflow. This webinar will cover: Features of iSeq 100, end-to-end workflow, the new Illumina technologies that power the iSeq 100, and the troubleshooting path when working with Technical Support.

  • iSeq 100: Support Resources

    In this video, we highlight the valuable user guides, videos, and online courses that will help you install and use your iSeq 100 Sequencing System.

    8 min

  • Sequencing Analysis Viewer (SAV): A Beginner's Guide

    Recorded Webinar (May 2020) | The Sequencing Analysis Viewer (SAV) Software is an application where users can view important quality metrics generated during sequencing runs. This webinar will provide a guided tour for beginners on how to use SAV, as well as tips and tricks for reviewing the most useful information for sequencing runs. This webinar is targeted at new users and will go over the following topics: how to load data into SAV, what metrics are available in each tab of the software, and understanding which are the most valuable metrics for run review and where to find them.

  • Library Prep


  • AmpliSeq for Illumina: Library Prep Protocol

    This course identifies the items needed for the AmpliSeq for Illumina library prep protocol, introduces pool planning resources for a multiplexed amplicon run, demonstrates the steps in the library prep protocol, and lists best practices for the protocol.

    30 min

  • AmpliSeq for Illumina: Overview

    This course describes the AmpliSeq for Illumina assay technology, lists the three types of panels, introduces the steps in the amplicon sequencing workflow, and lists the analysis options for amplicon sequencing data.

    15 min

  • Nextera DNA Flex Library Preparation

    By the end of this course, you will be able to identify the Nextera DNA Flex kit, describe how the protocol works, identify what is needed to complete the protocol, and define the various steps in the library preparation workflow.

    20 min

  • Amplicon Sequencing: Introduction

    Recorded Webinar (January 2020)| Illumina Technical Support invites you to join us to discuss the end-to-end workflow for an amplicon sequencing project. This webinar is ideal for new users of Illumina Next Generation Sequencing. We will discuss amplicon sequencing, including the following topics: what defines an amplicon library, kits and protocols for amplicon libraries, and amplicon sequencing runs and parameters.

  • Data Analysis


  • BaseSpace Sequence Hub: Introduction and Analysis Overview

    Recorded Webinar (May 2020) | llumina Technical Support invites you to learn about BaseSpace, Illumina’s cloud-based sequencing data analysis solution. In this presentation, we will discuss how to navigate the BaseSpace dashboard, general BaseSpace functionally, and tips and tricks for successful application and workflow utilization. We will cover the following topics: How to upload/view data in BaseSpace, discussion of major analysis workflows, general overview of how to analyze data on BaseSpace.

    Please note: BaseMount and BaseSpace CLI are alpha software releases; features and functionality may change over time, and support is directly through Illumina software development.

  • Diagnosing Suboptimal Clustering in Patterned Flow Cells

    Although over clustering is not possible on patterned flow cell, loading a library with a suboptimal concentration negatively impacts run data. This video discusses preventing and diagnosing common clustering issues on patterned flow cells.

    5 min

  • Local Run Manager 2.0 (Research Use Only): Introduction - On- and Off-Instrument

    Recorded Webinar (December 2019) | Local Run Manager is a Windows-based software for Illumina benchtop sequencing data that includes local analysis options as well as run and user management. This webinar targets new and intermediate users of the software. It will provide an overview of the Research Use Only (RUO) version of Local Run Manager 2, including the off-instrument version and the versions bundled with the latest MiSeq Control Software, iSeq Control Software, and NextSeq Control Software. This webinar does not cover the Dx versions of the software. We will cover the following topics: Features of Local Run Manager 2, available versions and analysis options, how to set up runs and analyze data, interaction with on-instrument control software, and comparison to other Illumina analysis software.

  • Optimal Cluster Density Best Practices

    This video discusses optimal cluster density and how over and under-clustering can affect your sequencing data. It also examines common clustering issues and ways to prevent them.

    10 min

  • Sequence Analysis Viewer (SAV): A Beginner's Guide (Spanish)

    Upcoming Webinar | El programa “Sequencing Analysis Viewer” (SAV) es una aplicación gratuita para ver importantes métricas de calidad generadas durante la secuenciación. Esta videoconferencia proporcionará una visita guiada para principiantes sobre cómo usar SAV, así como consejos para evaluar la información más pertinente al control de calidad. Esta presentación está dirigida a nuevos usuarios y cubrirá los siguientes temas: Cómo importar datos a SAV, Qué métricas están disponibles en cada pestaña del software, Comprender cuáles son las métricas más valiosas para la revisión de una corrida y dónde encontrarlas.

    Jul 22, 2020 PST