The Software version 1.6 is required to run the S Prime flow cells. This software is customer installable for systems already running version v1.4. Make sure to familiarize yourself with all changes noted in the Release Notes for v1.5 & v1.6 before upgrading the instrument software. To perform the upgrade, make sure that the system is on and the instrument is running. Log in to the administrator account on the NovaSeq, then download and unzip the executable. Run the software package from any location on the admin account.
If there custom options specified in the options .cfg file, transfer those values to the new options file in C:\ProgramData\Illumina\NovaSeq\NovaSeq Control Software\Configs.
Make sure that you manually power cycle the entire instrument to complete the installer tasks.