NovaSeq 6000 Documentation
NovaSeq 6000 System
Documentation
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System Documents
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DATE
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NovaSeq Xp Workflow Checklist
View ChecklistA condensed version of the NovaSeq Xp protocol for experienced users.
Dec 22, 2022
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NovaSeq Series Custom Primers Guide
DownloadInstructions for sequencing with custom primers on the NovaSeq Sequencing System.
< 1 MB
Jul 20, 2020
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Denature and Dilute Protocol Generator
Generate ProtocolInstructions for denaturing and diluting prepared libraries for sequencing.
Feb 28, 2025
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Supporting Documents
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DATE
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Cluster Optimization Overview
View Online HelpStrategies for optimizing cluster density and preventing and diagnosing clustering issues on Illumina flow cells.
Apr 26, 2021
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Sequencing Human Whole Genomes on the NovaSeq 6000 System
Information and recommendations for performing Human Whole-Genome Sequencing (WGS) on the NovaSeq 6000 Sequencing System.
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Indexed Sequencing Overview
View Online HelpThe sequencing process for single- and dual-indexed runs on Illumina instruments.
Aug 13, 2024
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Security and Networking
View Online HelpGuidelines for managing the security of the control computer on your sequencing system. Use these guidelines to configure your system and ensure a more secure operating environment.
It is strongly recommended that you review this documentation to keep your instrument secure.
Jan 12, 2024
Product Literature |
TYPE |
SIZE |
DATE |
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The NovaSeq 6000 System Specification SheetDownload |
Data sheet | ||
Human Whole-Genome Sequencing with the NovaSeq 6000 Sequencing SystemDownload |
Application note | < 1 MB | Aug 31, 2018 |
NovaSeq 6000 System Quality Scores and RTA3 SoftwareDownload |
Application note | < 1 MB | Oct 19, 2017 |
Sequencing Library QC on the iSeq SystemDownload |
Application note | < 1 MB | Nov 2, 2018 |
The NovaSeq Xp workflow provides flexibility and control without sacrificing data quality or yieldDownload |
Technical note | < 1 MB | Jan 15, 2019 |
QC and rebalancing of single-cell gene expression libraries using the iSeq™ 100 SystemDownload |
Application note | 2 MB | Jan 29, 2020 |
High-performance whole-genome sequencing with Illumina DNA PCR-Free Prep, TagmentationDownload |
Application note | < 1 MB | Jun 9, 2020 |
De Novo Genome Assembly with Illumina Sequencing and the NRGene DeNovoMAGIC SolutionDownload |
Application note | ||
NovaSeq 6000 Sequencing SystemDownload |
Data sheet | < 1 MB | Jul 26, 2022 |
NovaSeq 6000 Sequencing SystemDownload |
Data sheet | < 1 MB | Feb 7, 2022 |
Explore the transcriptome with single-cell resolutionDownload |
Technical note | 1 MB | Mar 1, 2022 |
Data Security with Illumina ProactiveDownload |
Technical note | < 1 MB | Oct 4, 2022 |
Impact of NovaSeq flow cell lane edge integrity on sequencing performanceDownload |
Technical note | 1 MB | Dec 15, 2021 |
Population genetics data processing with the DRAGEN Bio-IT PlatformDownload |
Technical note | 1 MB | |
Multiomic interrogation of the immune system at single-cell resolutionDownload |
Technical note | 1 MB | Mar 1, 2022 |
Sequencing performance with NovaSeq Control Software v1.8Download |
Technical note | < 1 MB | |
Unify single-cell gene expression and chromatin accessibilityDownload |
Technical note | 1 MB | |
Resolve the whole transcriptome within tissue architectureDownload |
Technical note | 3 MB | Jul 1, 2021 |
Enhanced sequencing capabilities with the NovaSeq™ 6000 v1.5 Reagent KitDownload |
Technical note | < 1 MB | Sep 30, 2020 |
Balancing sample coverage for whole-genome sequencingDownload |
Technical note | < 1 MB | Nov 20, 2024 |
Shotgun metagenomics with Illumina DNA Prep on the NovaSeq 6000 SystemDownload |
Application note | < 1 MB | |
Illumina Genomics Architecture enables PopGen studies with Illumina DNA PCR-Free PrepDownload |
Application note | 2 MB | Sep 7, 2023 |
High-plex spatial proteogenomics of FFPE tissue sectionsDownload |
Application note | 2 MB | |
Ultra-long range phasing with linked-read sequencing technologyDownload |
Application note | 1 MB | |
High-resolution, high-throughput spatial transcriptomics of complex tissuesDownload |
Application note | 3 MB | Aug 24, 2021 |