NovaSeq 6000 Documentation

  • Supporting Documents

    SIZE

    DATE


  • Cluster Optimization Overview

    Strategies for optimizing cluster density and preventing and diagnosing clustering issues on Illumina flow cells.

    View Online Help

    Apr 26, 2021


  • Sequencing Human Whole Genomes on the NovaSeq 6000 System

    Information and recommendations for performing Human Whole-Genome Sequencing (WGS) on the NovaSeq 6000 Sequencing System.

    View


  • Indexed Sequencing Overview

    The sequencing process for single- and dual-indexed runs on Illumina instruments.

    View Online Help

    Apr 26, 2021


  • Security and Networking

    Guidelines for managing the security of the control computer on your sequencing system. Use these guidelines to configure your system and ensure a more secure operating environment.

    It is strongly recommended that you review this documentation to keep your instrument secure.

    View Online Help

    Jan 12, 2024


Product Literature

TYPE

SIZE

DATE

The NovaSeq 6000 System Specification Sheet
Download
Data sheet
Human Whole-Genome Sequencing with the NovaSeq 6000 Sequencing System
Download
Application note < 1 MB Aug 31, 2018
NovaSeq 6000 System Quality Scores and RTA3 Software
Download
Application note < 1 MB Oct 19, 2017
Sequencing Library QC on the iSeq System
Download
Application note < 1 MB Nov 2, 2018
The NovaSeq Xp workflow provides flexibility and control without sacrificing data quality or yield
Download
Technical note < 1 MB Jan 15, 2019
QC and rebalancing of single-cell gene expression libraries using the iSeq™ 100 System
Download
Application note 2 MB Jan 29, 2020
High-performance whole-genome sequencing with Illumina DNA PCR-Free Prep, Tagmentation
Download
Application note < 1 MB Jun 9, 2020
De Novo Genome Assembly with Illumina Sequencing and the NRGene DeNovoMAGIC Solution
Download
Application note
NovaSeq 6000 Sequencing System
Download
Data sheet < 1 MB Jul 26, 2022
NovaSeq 6000 Sequencing System
Download
Data sheet < 1 MB Feb 7, 2022
Explore the transcriptome with single-cell resolution
Download
Technical note 1 MB Mar 1, 2022
Data Security with Illumina Proactive
Download
Technical note < 1 MB Oct 4, 2022
Impact of NovaSeq flow cell lane edge integrity on sequencing performance
Download
Technical note 1 MB Dec 15, 2021
Population genetics data processing with the DRAGEN Bio-IT Platform
Download
Technical note 1 MB
Multiomic interrogation of the immune system at single-cell resolution
Download
Technical note 1 MB Mar 1, 2022
Sequencing performance with NovaSeq Control Software v1.8
Download
Technical note < 1 MB
Unify single-cell gene expression and chromatin accessibility
Download
Technical note 1 MB
Resolve the whole transcriptome within tissue architecture
Download
Technical note 3 MB Jul 1, 2021
Enhanced sequencing capabilities with the NovaSeq™ 6000 v1.5 Reagent Kit
Download
Technical note < 1 MB Sep 30, 2020
Balancing sample coverage for whole-genome sequencing
Download
Technical note < 1 MB Apr 5, 2021
Long read lengths for shotgun metagenomics
Download
Application note < 1 MB Aug 12, 2021
Illumina Genomics Architecture enables PopGen studies with Illumina DNA PCR-Free Prep
Download
Application note 2 MB Sep 7, 2023
High-plex spatial proteogenomics of FFPE tissue sections
Download
Application note 2 MB
Ultra-long range phasing with linked-read sequencing technology
Download
Application note 1 MB
High-resolution, high-throughput spatial transcriptomics of complex tissues
Download
Application note 3 MB Aug 24, 2021