NovaSeq 6000 Training

  • Introductory Topics


  • Bcl2Fastq v2+: Introduction

    Recorded Webinar (April 2019) | Bcl2Fastq is a Linux-based software that converts base call files generated from an Illumina sequencing run to FASTQ files as well as demultiplex samples. This webinar focuses on Bcl2fastq v2+ and is targeted for new to intermediate users who are interested in the configurable options that this command line program allows. We will cover the following topics: requirements to run Bcl2Fastq, command line basics, commonly used options, frequently asked ‘How To….’ questions.

  • How Do I Prepare for My Illumina Instrument Delivery?

    In this video, an Illumina Field Applications Scientist discusses what to expect during the pre-installation, shipping, and delivery stages. We’ll highlight important steps in the process and introduce you to available resources. Note that, depending on your region, the steps shown in this video may not apply to the iSeq 100 system.

    7 min

  • NovaSeq 6000: Introduction

    Recorded Webinar (February 2021) | Join Illumina Technical Support for a discussion on the NovaSeq 6000 platform. This webinar is targeted towards users with a basic understanding of the Illumina sequencing workflow. We will cover the following topics: features of NovaSeq 6000, end-to-end workflow, library preparation considerations, guidance on run analysis, and tips and best practices for running and maintaining your instrument.

  • NovaSeq 6000: Preparing for Your Install

    This course introduces lab requirements, computing requirements, and delivery considerations for the NovaSeq 6000 System. It also discusses the consumables required for running and maintaining the instrument.

    15 min

  • NovaSeq 6000: System Overview
    This course introduces the features of the NovaSeq 6000, the steps in the sequencing workflow, and the NovaSeq sequencing technology.

    20 min

  • Sequencing Analysis Viewer (SAV): A Beginner's Guide

    Recorded Webinar (December 2020) | The Sequencing Analysis Viewer (SAV) Software is an application where users can view important quality metrics generated during sequencing runs. This webinar will provide a guided tour for beginners on how to use SAV, as well as tips and tricks for reviewing the most useful information for sequencing runs. This webinar is targeted at new users and will go over the following topics: how to load data into SAV, what metrics are available in each tab of the software, and understanding which are the most valuable metrics for run review and where to find them.

  • Sequencing: Introduction to Sequencing By Synthesis (SBS)

    Recorded Webinar (January 2021) | Illumina next-generation sequencing technology allows for massive parallel sequencing. Illumina Technical Support invites you to join us for a presentation and discussion on sample preparation, cluster generation and sequencing by synthesis chemistry. This webinar is targeted to those new to next-generation sequencing and will discuss the following topics: Illumina library construction, cluster generation methods by platform, sequencing by synthesis, and multiplexing and primary analysis.

  • Instruments & Software


  • Diagnosing Suboptimal Clustering in Patterned Flow Cells

    Although over clustering is not possible on patterned flow cell, loading a library with a suboptimal concentration negatively impacts run data. This video discusses preventing and diagnosing common clustering issues on patterned flow cells.

    5 min

  • Discover the Benefits of Illumina Proactive

    Learn about the benefits of instrument performance monitoring through Illumina Proactive, a service in which instrument performance data are sent to Illumina to enable proactive maintenance.

    10 min

  • IT Requirements for Implementing Illumina Proactive

    This video shares IT requirements for implementing Illumina Proactive, an instrument performance monitoring service provided by Illumina that can increase instrument uptime, improve operational efficiency, and reduce the risk of lost resources.

    4 min

  • NovaSeq 6000: How to Start a Run
    This course introduces the steps to start a NovaSeq 6000 sequencing run, prepare the consumables, and use the NovaSeq Control Software.

    20 min

  • NovaSeq: Xp Workflow

    This video demonstrates the NovaSeq™ Xp workflow, including NovaSeq flow cell preparation, ExAmp and library master mix preparation, and manually loading libraries onto the flow cell.

    10 min

  • Optimal Cluster Density Best Practices

    This video discusses optimal cluster density and how over and under-clustering can affect your sequencing data. It also examines common clustering issues and ways to prevent them.

    10 min