Kit Requirements & Contents

NovaSeq 6000 Kit Requirements

Performing a single flow cell run on the NovaSeq Sequencing System requires 1 single-use NovaSeq 6000 Reagent Kit. For a dual flow cell run, use 2 kits.

Requirements & Compatibility

Flow Cell Types

Use the appropriate reagent cartridges for a given flow cell type. 

Kit Name

Flow Cell Type

NovaSeq 6000 S4 Reagent Kit

NovaSeq 6000 S4 Flow Cell

NovaSeq 6000 S2 Reagent Kit

NovaSeq 6000 S2 Flow Cell

NovaSeq 6000 S1 Reagent Kit

NovaSeq 6000 S1 Flow Cell

NovaSeq 6000 Kit Contents

The flow cell is stored at 2°C to 8°C. Before unpackaging, allow the unopened flow cell package to reach room temperature.Contents & Storage




Storage Temperature Preparation


Buffer Cartridge

Contains sequencing buffers

15°C to 30°C Use directly from storage


Cluster Cartridge Contains clustering, indexing, and paired-end reagents -25°C to -15°C Thaw ~4 hours using a water bath.*
1 SBS Cartridge Contains sequencing reagents -25°C to -15°C
Thaw ~4 hours using a water bath.*
1 Flow Cell Patterned flow cell 2°C to 8°C Allow unopened flow cell to reach room temperature. 
1 Library Tube Contains pooled and denatured libraries 2°C to 8°C  

*Alternatively, thaw overnight using a combination of refrigeration, room temperature, and water bath methods.

Safety Data Sheets

See Safety Data Sheets.