NovaSeq 6000 Reagent Kits

Catalog IDs: 20027464, 20027465, 20029137, 20012865, 20012864, 20012863, 20012862, 20012861, 20012860, 20027466, 20012866

Kit Requirements

Performing a single flow cell run requires a single-use NovaSeq Reagent Kit. For a dual flow cell run, use two kits.

For other system requirements and product compatibility, see Compatible Products.

Flow Cell Types

Use the appropriate reagent cartridges for a given flow cell type.

Kit Name

Flow Cell Type

NovaSeq 6000 S4 Reagent Kit

NovaSeq 6000 S4 Flow Cell

NovaSeq 6000 S2 Reagent Kit

NovaSeq 6000 S2 Flow Cell

NovaSeq 6000 S1 Reagent Kit

NovaSeq 6000 S1 Flow Cell

Contents and Storage

Reagents in the SBS and buffer cartridge are sensitive to light. Store the cartridges in a dark location away from light.

The flow cell is stored at 2°C to 8°C. Before unpackaging, allow the unopened flow cell package to reach room temperature.Contents & Storage




Storage Temperature


Buffer Cartridge

Contains sequencing buffers

15°C to 30°C


Cluster Cartridge Contains clustering, indexing, and paired-end reagents -25°C to -15°C
1 SBS Cartridge Contains sequencing reagents -25°C to -15°C
1 Flow Cell Patterned flow cell 2°C to 8°C
1 Library Tube Contains pooled and denatured libraries 2°C to 8°C