Local Run Manager Compatible Libraries

Custom Libraries

Most analysis modules prompt you to select a library prep kit when creating a run in Local Run Manager. You can create a custom library prep kit definition for use with an analysis module using tools on the Local Run Manager dashboard. See the Local Run Manager Software Guide and example kit definition files.

Supported Libraries

DNA Amplicon Analysis Module

The Amplicon analysis module aligns reads against the reference specified in the manifest file, and then performs variant analysis.

DNA Enrichment Analysis Module

The DNA Enrichment analysis module aligns reads against the whole genome reference, and then performs variant analysis for regions of interest specified in the manifest file.

Library Kit Category Library Kit Name
Nextera Rapid Capture Enrichment Nextera Rapid Capture
TruSight Enrichment Panels TruSight Rapid Capture, including:
  • TruSight Autism
  • TruSight Cancer
  • TruSight Inherited Disease
Generate FASTQ Analysis Module

The Generate FASTQ analysis module demultiplexes indexed reads, generates intermediate analysis files in the FASTQ file format, and then exits the workflow. FASTQ files are input for third-party analysis tools.

Library Kit Category Library Kit Name
TruSeq Amplicon

TruSeq Amplicon - Cancer Panel

TruSeq Custom Amplicon 1.5

TruSeq Custom Amplicon Low Input

TruSight Amplicon Panels TruSight Myeloid Panel
Nextera Nextera DNA
Nextera Mate Pair Nextera Mate Pair
Nextera XT V2 Nextera XT with Index Kits v2
Nextera XT Nextera XT
TruSeq HT

TruSeq Nano DNA HT

TruSeq DNA PCR-Free HT

TruSeq LT

TruSeq Nano DNA LT

TruSeq DNA PCR-Free LT

Nextera Rapid Capture Enrichment Nextera Rapid Capture
TruSight Enrichment Panels

TruSight One

TruSight Rapid Capture, including:

  • TruSight Autism
  • TruSight Cancer
  • TruSight Inherited Disease
TruSeq Small RNA TruSeq Small RNA
RNA Amplicon Analysis Module

The RNA Amplicon analysis module aligns reads specified in the manifest file, quantifies the relative expression of genes and isoforms between samples, and then compares abundance across samples.

TruSight Tumor 15 Analysis Module

The TruSight Tumor 15 analysis module aligns reads specified in the manifest file, and then reports somatic variants of a set of reference panel genes associated with cancer.

By default, the analysis module is set to TruSight Tumor 15 libraries.

VeriSeq PGS Analysis Module

The VeriSeq PGS analysis module demultiplexes and aligns reads to the human reference genome. BAM files are generated for use with BlueFuse™ Multi Analysis Software.

By default, the analysis module is set to VeriSeq PGS libraries.