Compatible Libraries for Analysis Modules

Analysis Modules

For most analysis modules, you are prompted to select a library prep kit used for the run when using the Create Run feature in Local Run Manager. The options list library prep kits by kit category. The information on this page lists compatible library prep kits for each available kit category.

You can create a custom library prep kit definition for use with an analysis module using tools on the Local Run Manager dashboard. See the Local Run Manager Software Guide and example kit definition files.

DNA Amplicon Analysis Module

The Amplicon analysis module aligns reads against the reference specified in the manifest file, and then performs variant analysis.

Compatible Libraries

DNA Enrichment Analysis Module

The DNA Enrichment analysis module aligns reads against the whole genome reference, and then performs variant analysis for regions of interest specified in the manifest file.

Compatible Libraries

Library Kit Category

Library Kit Name

Nextera Rapid Capture Enrichment

Nextera Rapid Capture

TruSight Enrichment Panels

TruSight Rapid Capture, including:
  • TruSight Autism
  • TruSight Cancer
  • TruSight Inherited Disease

Generate FASTQ Analysis Module

The Generate FASTQ analysis module demultiplexes indexed reads, generates intermediate analysis files in the FASTQ file format, and then exits the workflow. FASTQ files are input for third-party analysis tools.

Compatible Libraries

Library Kit Category

Library Kit Name

TruSeq Amplicon

TruSeq Amplicon - Cancer Panel

TruSeq Custom Amplicon 1.5

TruSeq Custom Amplicon Low Input

TruSight Amplicon Panels

TruSight Myeloid Panel

Nextera

Nextera DNA

Nextera Mate Pair

Nextera Mate Pair

Nextera XT V2

Nextera XT with Index Kits v2

Nextera XT

Nextera XT

TruSeq HT

TruSeq Nano DNA HT

TruSeq DNA PCR-Free HT

TruSeq LT

TruSeq Nano DNA LT

TruSeq DNA PCR-Free LT

Nextera Rapid Capture Enrichment

Nextera Rapid Capture

TruSight Enrichment Panels

TruSight One

TruSight Rapid Capture, including:

  • TruSight Autism
  • TruSight Cancer
  • TruSight Inherited Disease

TruSeq Small RNA

TruSeq Small RNA

RNA Amplicon Analysis Module

The RNA Amplicon analysis module aligns reads specified in the manifest file, quantifies the relative expression of genes and isoforms between samples, and then compares abundance across samples.

Compatible Libraries

TruSight Tumor 15 Analysis Module

The TruSight Tumor 15 analysis module aligns reads specified in the manifest file, and then reports somatic variants of a set of reference panel genes associated with cancer.

Compatible Libraries

By default, the analysis module is set to TruSight Tumor 15 libraries.