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Training
Introductory Courses
| Online Course Title | Description | Length |
| MiSeq: Sequencing Chemistry | After completing this course you will be able to describe the Illumina sequencing workflow, discuss the clustering process, and define the sequencing process. Languages: Spanish, Chinese (Simplified), Japanese |
20 min |
| MiSeq: Sequencing Fundamentals | After completing this course you will be able to describe DNA, RNA and the central dogma; discuss traditional sequencing methodologies; and compare traditional sequencing to Illumina's sequencing chemistry. |
15 min |
| Sequencing: Illumina Technology | This course provides a general overview of the Illumina sequencing workflow from extraction of nucleic acids to the completion of a sequencing run. Languages: Spanish, Chinese (Simplified) |
30 min |
| Sequencing: Illumina's Dual Indexing Strategy | The goal of dual index sequencing is to increase the multiplex level of sequencing per lane, so that more samples can be sequenced on the same flow cell. Languages: Spanish, Chinese (Simplified), Japanese |
10 min |
Sample Prep
| Online Course Title | Description | Length |
| DesignStudio: TruSeq Targeted RNA Expression | After completing this course you will be able to create a project using DesignStudio Gene Expression. Languages: Spanish, Chinese (Simplified), Japanese |
10 min |
| Illumina Experiment Manager | This course provides an introduction to Illumina Experiment Manager, and shows you the steps to create sample plates and sample sheets. |
25 min |
| Nextera DNA Sample Prep | After completing this course you will be able to describe the items necessary to run Nextera DNA sample preparation assay to generate MiSeq sequence-ready libraries, describe the steps in Nextera DNA sample preparation workflow, and identify some of the assay's most important considerations. Languages: Spanish, Chinese (Simplified), Japanese |
20 min |
| Nextera Rapid Capture | After completing this course, you will be able to identify the Nextera Rapid Capture Enrichment kits, choose your Nextera Rapid Capture Enrichment Product, identify how the protocol works, describe what is needed to successfully complete the protocol, define the various steps in the Sample Preparation workflow, and analyze Nextera Capture Enrichment data. Languages: Spanish, Chinese (Simplified), Japanese |
20 min |
| Nextera Sample Prep: Best Practices | After completing this course you will be able to determine appropriate starting material, perform critical techniques used in the sample prep process, and accurately validate the final libraries.
|
30 min |
| TruSeq Amplicon - Cancer Panel | By the end of this training, you will be able to describe the items necessary to run the TruSeq Amplicon Cancer Panel assay, describe the steps in the sequencing workflow, identify some of the best practices, and set up a sample sheet for sequencing.
|
20 min |
| TruSeq Targeted RNA Expression Kit | After completing this course you will be able to describe the TruSeq Targeted RNA Expression Kit, the steps to order a custom oligo pool, the steps in the TruSeq Targeted RNA Expression protocol, best practices, and how to analyze and normalize data. Languages: Spanish, Chinese (Simplified), Japanese |
20 min |
| TruSeq: Best Practices | After completing this course you will understand best practices for all TruSeq Sample Preparation Kits including liquid handling, AMPure XP handling, avoiding cross-contamination, and temperature considerations. You will also learn about specific information about DNA and RNA best practices. Languages: Spanish, Chinese (Simplified), Japanese |
20 min |
| TruSeq: Comparison of Available DNA Sample Preparation Kits | After completing this course you will know the similarities and differences between currently available TruSeq DNA Sample Prep Kits, the starting input amounts and final library characteristics for the various kits, how the various protocols differ, and the ordering information for the various TruSeq DNA kits.
|
20 min |
| TruSeq: Controls | By the end of this course you will be able to identify controls, describe the different SAV reporting types, and understand how to support these controls. Languages: Spanish, Chinese (Simplified), Japanese |
15 min |
| TruSeq: DNA PCR-Free Library Prep | Topics included in this course include the TruSeq DNA PCR-Free library prep protocol, what the protocol does, how the protocol works, what is needed to complete the protocol successfully, and the various steps in the workflow. Languages: Spanish, Chinese (Simplified), Japanese |
20 min |
| TruSeq: DNA Sample Prep | After completing this course you will know the TruSeq DNA Sample Prep Protocol, what the protocol does, how the protocol works, what is needed to successfully complete the protocol, and the various steps in the Sample Prep Workflow.
|
10 min |
| TruSeq: Nano DNA Sample Prep | After completing this course, you will know: what the TruSeq Nano DNA Sample Prep Protocol is, what the protocol does, how the protocol works, what is needed to successfully complete the protocol, and the various steps in the sample prep workflow. Languages: Spanish, Chinese (Simplified), Japanese |
20 min |
| TruSeq: Sample Purification Bead Size Selection and Best Practices | After completing this course you will understand what Sample Purification Beads are, how the Sample Purification Beads work, when Sample Purification Beads are used, what the Size Selection Workflow is, and the Sample Purification Beads best practices.
|
20 min |
| TruSeq: Small RNA Sample Prep | After completing this course you will know the TruSeq Small RNA Sample Prep Protocol, what the protocol does, how the protocol works, what is needed to successfully complete the protocol, and the various steps in the Sample Prep Workflow. Languages: Spanish, Chinese (Simplified), Japanese |
10 min |
| TruSeq: Stranded RNA Sample Prep | After completing this course, you will learn answers to questions including: What is the TruSeq Stranded mRNA Sample Prep Protocol? and What is the TruSeq Stranded Total RNA Sample Prep Protocol Languages: Spanish, Chinese (Simplified), Japanese |
20 min |
| DesignStudio: TruSeq Custom Amplicon | This course provides an introduction to the DesignStudio application by walking the user through ordering a experiment. Languages: Japanese |
20 min |
| TruSeq: Custom Amplicon Library Prep | After completing this course you will be able to describe the items necessary to run the TruSeq Custom Amplicon assay, list the steps in the workflow, and identify the best practices. Languages: Spanish, Chinese (Simplified), Japanese |
20 min |
Instruments & Software
| Online Course Title | Description | Length |
| MiSeq: Getting Started | After completing this course you will be able to describe the lab environment required to use MiSeq, list key components of MiSeq, and list the user-supplied consumables required for running and maintaining MiSeq. Languages: Spanish, Chinese (Simplified), Japanese |
20 min |
| MiSeq: How to Start a Run | After completing this course you will be able to list the library construction methods available for MiSeq, create an electronic sample sheet, prepare and load MiSeq reagents, and start a sequencing run using MiSeq Control Software (MCS). Languages: Spanish, Chinese (Simplified), Japanese |
20 min |
| MiSeq: Instrument Washes | This course provides an overview of the three MiSeq instrument washes: the post-run wash, the maintenance wash, and the standby wash. Languages: Spanish, Chinese (Simplified), Japanese |
10 min |
| MiSeq: Introduction to the MiSeq System | After completing this course, you will be able to describe the simplified sequencing workflow on the MiSeq, understand the features of MiSeq, and list the six MiSeq sequencing workflows. Languages: Spanish, Chinese (Simplified), Japanese |
20 min |
Data Analysis
| Online Course Title | Description | Length |
| MiSeq: Data Analysis Overview | After completing this course you will be able to list the MiSeq data analysis tools, briefly describe the function of each tool, and list some considerations when choosing your MiSeq data analysis tool. |
10 min |
| MiSeq: Does My Run Look Good? | After completing this course you will be able to list two options for monitoring your MiSeq run, describe key metrics in MiSeq Control Software (MCS), and describe key metrics in Sequencing Analysis Viewer (SAV). |
10 min |
| MiSeq: Imaging and Base Calling | After completing this course you will have an understanding of how the MiSeq processes images to extract intensities and processes intensities to extract base calls. These processes are completed automatically by the MiSeq. Languages: Spanish, Chinese (Simplified), Japanese |
35 min |
| MiSeq: MiSeq Reporter | After completing this course you will have an understanding of the demultiplexing and alignment processes and program interfaces that are common to all workflows in MiSeq Reporter. You will also be able to briefly describe the utility of the supported MiSeq Reporter workflows. Languages: Spanish, Chinese (Simplified), Japanese |
25 min |
| BaseSpace Sequence Hub: FAQs | This course helps you to locate answers to commonly asked questions about BaseSpace Sequence Hub. |
25 min |
| BaseSpace Sequence Hub: Introduction | The course explores the BaseSpace Sequence Hub interface and its key features. |
20 min |