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Training
Introductory Courses
| Online Course Title | Description | Length |
| Sequencing: Illumina Technology | This course provides a general overview of the Illumina sequencing workflow from extraction of nucleic acids to the completion of a sequencing run. |
15 min |
| Sequencing: Fundamentals | After completing this course you will be able to describe DNA, RNA and the central dogma; discuss traditional sequencing methodologies; and compare traditional sequencing to sequencing by synthesis. |
15 min |
Library Prep
| Online Course Title | Description | Length |
| Illumina Experiment Manager | This course provides an introduction to Illumina Experiment Manager, and shows you the steps to create sample plates and sample sheets. |
25 min |
| AmpliSeq for Illumina: Library Prep Protocol | This course identifies the items needed for the AmpliSeq for Illumina library prep protocol, introduces pool planning resources for a multiplexed amplicon run, demonstrates the steps in the library prep protocol, and lists best practices for the protocol. |
30 min |
| AmpliSeq for Illumina: Overview | This course describes the AmpliSeq for Illumina assay technology, lists the three types of panels, introduces the steps in the amplicon sequencing workflow, and lists the analysis options for amplicon sequencing data. |
15 min |
| Nextera DNA Flex Library Preparation | By the end of this course, you will be able to identify the Nextera DNA Flex kit, describe how the protocol works, identify what is needed to complete the protocol, and define the various steps in the library preparation workflow. |
20 min |
Instruments & Software
| Online Course Title | Description | Length |
| Local Run Manager | This course shows you how to get started using the Local Run Manager software to set up your sequencing runs and view analysis results. |
20 min |
| MiSeq: How to Start a Run | After completing this course you will be able to identify the MiSeq run setup options, describe how to prepare the MiSeq reagent cartridge and load libraries, and list the steps to start a sequencing run with MiSeq Control Software (MCS). |
20 min |
| MiSeq: Instrument Washes | This course provides an overview of the three MiSeq instrument washes: the post-run wash, the maintenance wash, and the standby wash. Languages: Spanish, Chinese (Simplified), Japanese |
10 min |
| MiSeq: Introduction to the MiSeq System | After completing this course, you will be able to describe the simplified sequencing workflow on the MiSeq, understand the features of MiSeq, and list the six MiSeq sequencing workflows. Languages: Spanish, Chinese (Simplified), Japanese |
20 min |
| MiSeq: Preparing for Your Install | After completing this course you will be able to describe the lab environment required to use MiSeq, list key components of MiSeq, identify the network considerations for MiSeq, and list the user-supplied consumables required for running and maintaining MiSeq. |
20 min |
Data Analysis
| Online Course Title | Description | Length |
| MiSeq: Data Analysis Overview | After completing this course you will be able to list the MiSeq data analysis tools, briefly describe the function of each tool, and list some considerations when choosing your MiSeq data analysis tool. |
10 min |
| MiSeq: Does My Run Look Good? | After completing this course you will be able to list two options for monitoring your MiSeq run, describe key metrics in MiSeq Control Software (MCS), and describe key metrics in Sequencing Analysis Viewer (SAV). |
10 min |
| MiSeq: Imaging and Base Calling | After completing this course you will have an understanding of how the MiSeq processes images to extract intensities and processes intensities to extract base calls. These processes are completed automatically by the MiSeq. Languages: Spanish, Chinese (Simplified), Japanese |
35 min |
| MiSeq: MiSeq Reporter | After completing this course you will have an understanding of the demultiplexing and alignment processes and program interfaces that are common to all workflows in MiSeq Reporter. You will also be able to briefly describe the utility of the supported MiSeq Reporter workflows. Languages: Spanish, Chinese (Simplified), Japanese |
25 min |