MiSeq i100 Series Training

These free self-paced courses guide you through setting up and getting started with your MiSeq i100 system. If you are new to Illumina and next-generation sequencing, see the NGS for beginners page for additional resources.
The Illumina service and support team offers paid services for instrument installation, qualification, and training. Contact your sales representative for more information.
  • Introductory


  • Sequencing: Illumina Technology

    Course duration: 15 minutes | This course provides a general overview of the Illumina sequencing workflow from extraction of nucleic acids to the completion of a sequencing run.


  • Instrument and Software


  • MiSeq i100 Series: Setup

    Course duration: 20 minutes | This course shows you how to set up your MiSeq i100 Series system. Topics include preparing your lab space, identifying the test components, and performing first time setup.


  • MiSeq i100 Series: How to start a run

    Course duration: 25 minutes | Learn how to start a run on the MiSeq i100 Series. This course introduces the options for planning a run and analyzing sequencing data, consumable preparation, and how to use the MiSeq i100 Series Control Software to initiate the run.


  • MiSeq i100 Series: Does my run look good?

    Course duration: 20 minutes | Find out about the options for monitoring and assessing your MiSeq i100 Series run, including the available options, where to find key metrics, and some troubleshooting steps if your run does not meet expectations.


  • Library Prep


  • Illumina DNA Prep

    Course duration: 20 minutes | Illumina DNA prep is a fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications. Topics in this course include the components of the kit, how the protocol works, what is needed to complete the protocol, and various steps in the library preparation workflow.


  • Illumina DNA Prep with Enrichment

    Course duration: 30 minutes | Illumina DNA Prep with Enrichment is a complete targeted resequencing solution for a wide range of applications, with fast integrated workflow and options for custom and fixed enrichment panels. Learn about the kit and its components, how the protocol works, and various steps in the library preparation workflow.


  • Illumina RNA Prep with Enrichment

    Course duration: 30 minutes | Illumina RNA Prep with Enrichment is a fast, integrated workflow for producing enriched and indexed sequencing libraries from a broad range of sample types and RNA input quantities. This course describes the kit and its components, summarizes the steps of the protocol, and provides best practices.


  • Applications


  • MiSeq i100 Series: BCL Convert workflow

    Course duration: 25 minutes | This course provides an overview of the BCL convert workflow on the MiSeq i100 Series. Learn about the features of the workflow, steps in the workflow, and the steps to analyze data with the onboard DRAGEN BCL convert app.


  • MiSeq i100 Series: Library QC workflow

    Course duration: 25 minutes | This course provides an overview of the Library QC workflow on the MiSeq i100 Series. We'll cover the features of the Library QC workflow, the steps in the workflow, and review the onboard DRAGEN Library QC app analysis results.


  • MiSeq i100 Series: Microbial enrichment panel analysis workflow

    Course duration: 30 minutes | This course provides an overview of the microbial enrichment panel analysis workflow with the MiSeq i100 Series. Topics covered include features of the workflow, steps in the workflow, the available enrichment panels, and the steps to analyze the data with the onboard DRAGEN Microbial Enrichment Plus app.


  • MiSeq i100 Series: Small WGS workflow

    Course duration: 20 minutes | Learn about the Small Whole Genome Sequencing (Small WGS) workflow on the MiSeq i100 Series. We'll introduce features of the Small WGS workflow, describe the steps in the workflow, and review the results of the onboard DRAGEN Small WGS app.