With the release of version 1.2.0 of the BaseSpace™ Sequence Hub Command Line Interface (CLI) tool, users can now upload runs to BaseSpace™ Sequence Hub after sequencing has already completed. This tutorial covers setting proper permissions and using BaseSpace CLI to upload a run to your BaseSpace™ account. For more information and help getting started with BaseSpace™ CLI, see the links below: Getting Started - BaseSpace™ Command Line Interface (CLI): https://www.youtube.com/watch?v=WLAjo9aT7Ms Support Bulletin - How to use BaseSpace™ Command Line Interface (CLI) to upload run folders to BaseSpace Sequence Hub: https://support.illumina.com/bulletins/2020/07/how-to-use-basespace--command-line-interface--cli--to-upload-run.html BaseSpace™ CLI Overview: https://developer.basespace.illumina.com/docs/content/documentation/cli/cli-overview BaseSpace™ CLI Example Commands: https://developer.basespace.illumina.com/docs/content/documentation/cli/cli-examples Direct Download Links: Linux: https://api.bintray.com/content/basespace/BaseSpaceCLI-EarlyAccess-BIN/latest/\$latest/amd64-linux/bs?bt_package=latest Mac OS: https://api.bintray.com/content/basespace/BaseSpaceCLI-EarlyAccess-BIN/latest/\$latest/amd64-osx/bs?bt_package=latest Windows: https://api.bintray.com/content/basespace/BaseSpaceCLI-EarlyAccess-BIN/latest/\$latest/amd64-windows/bs.exe?bt_package=latest The methods and tools depicted in this video are for research use only and not for use in diagnostic procedures. ©2020 Illumina, Inc. All Rights Reserved. QB #10284 Subscribe to the Illumina video channel http://www.youtube.com/subscription_center?add_user=IlluminaInc A global genomics leader, Illumina provides comprehensive next-generation sequencing solutions to the research, clinical, and applied markets. Illumina technology is responsible for generating more than 90% of the world’s sequencing data.* Through collaborative innovation, Illumina is fueling groundbreaking advancements in oncology, reproductive health, genetic disease, microbiology, agriculture, forensic science, and beyond. *Data calculations on file. Illumina, Inc., 2015. View customer spotlight videos https://www.youtube.com/playlist?list=PLKRu7cmBQlajfheLzgbI4S7xBn7IDbt79 View Illumina webinars https://www.youtube.com/playlist?list=PLKRu7cmBQlahpXlnrrXlQw9itVJ8yHwUZ View Illumina product videos https://www.youtube.com/playlist?list=PLKRu7cmBQlaj6YuZmkfxZcT9twqDgP2Xd View Illumina support videos https://www.youtube.com/playlist?list=PLKRu7cmBQlajbm2KGsICWb-JOnusJfYvM