Illumina Genomics Research

The Illumina DRAGEN secondary analysis pipeline has been established as a trusted, comprehensive, accurate, and fast solution that helps NGS users retrieve maximal information out of their data. DRAGEN analysis onboard the NovaSeq X offers a unique, cost-effective way for users to analyze the enormous amount of data generated.

In this article, we highlight the improvements to sequencing and calibration workflows that enable the NovaSeq X to deliver 85% of bases in the highest quality bin (Q40) with excellent correlation to empirical accuracy; the use of PCR-free library preps in the Q-score calibration process; and the data management and cost benefits of quantizing the Q scores to three bins.

The UPIP Enrichment Kit was designed for broad, accurate detection of microbial and AMR sequences. To examine its performance in wastewater AMR surveillance, we sequenced wastewater samples with and without UPIP enrichment on the NovaSeq 6000 System, generating on average 300 million total reads per sample, and compared the AMR yield across different downsampled sequencing depths.