This video presents the Illumina Infinium™ assay lab workflow for HD, HTS, and LCG format BeadChips™ and is intended as a visual introduction and reference for new Infinium users. • Protocol steps demonstrated: Amplification, Fragmentation, Precipitation, Resuspension, BeadChip™ Hybridization, Washing and XStain, and Scanning. • Key techniques and Best practices highlighted: Decanting the amplification plate, Removing the cover seal, Maintaining the Glass Back Plates, and Assembling the flow through chamber. See the Infinium Assay Lab Setup and Procedures Guide here: https://support.illumina.com//downloads/infinium-assay-lab-setup-and-procedures-11322460.html To learn more about selecting BeadChips, required equipment, setting up your lab, and data analysis, watch the “Discover the Flexible, Scalable Infinium Workflow” here: https://youtu.be/kkRgC_PwaFY QB 11404 Subscribe to the Illumina video channel http://www.youtube.com/subscription_center?add_user=IlluminaInc A global genomics leader, Illumina provides comprehensive next-generation sequencing solutions to the research, clinical, and applied markets. Illumina technology is responsible for generating more than 90% of the world’s sequencing data.* Through collaborative innovation, Illumina is fueling groundbreaking advancements in oncology, reproductive health, genetic disease, microbiology, agriculture, forensic science, and beyond. *Data calculations on file. Illumina, Inc., 2015. View customer spotlight videos https://www.youtube.com/playlist?list=PLKRu7cmBQlajfheLzgbI4S7xBn7IDbt79 View Illumina webinars https://www.youtube.com/playlist?list=PLKRu7cmBQlahpXlnrrXlQw9itVJ8yHwUZ View Illumina product videos https://www.youtube.com/playlist?list=PLKRu7cmBQlaj6YuZmkfxZcT9twqDgP2Xd View Illumina support videos https://www.youtube.com/playlist?list=PLKRu7cmBQlajbm2KGsICWb-JOnusJfYvM TIMESTAMPS 00:00 Infinium™ Protocol Video 00:10 Amplification Step 00:57 Fragmentation 01:52 Precipitation 04:13 Resuspension 04:56 BeadChip™ Hybridization 07:21 Infinium Technique: Removing the Cover Seal 08:17 Infinium Technique: Assembling the Flow-Through Chamber 09:55 Best Practice: Maintaining the Glass Back Plates 10:55 XStain, with degassing Te-Flow 14:25 Scanning/Imaging