Low-Quality/FFPE RNA-Seq Solutions

mRNA sequencing (mRNA-Seq) is an accurate and cost-effective method for analyzing the coding transcriptome. Focusing your sequencing budget on only the messenger RNAs allows for increased sample throughput and lower per sample costs relative to total RNA-Seq, while enabling discovery of novel features such as alternative splicing, fusion transcripts and coding variants.^1,2

RNA Exome Capture Sequencing overcomes the limitation of traditional poly-A-based methods for capturing the coding transcriptome; these traditional methods are ineffective for FFPE RNA-Seq analysis. By applying sequence-specific capture that does not rely on the presence of polyadenylated transcripts, this method is ideal for mRNA-Seq with FFPE or degraded samples and samples with limited starting material.

Total RNA sequencing provides a comprehensive view of the transcriptome, enabling analysis of both coding and multiple forms of noncoding RNA in a single experiment.

This method provides highly accurate transcript abundance information, and can detect novel features such as gene fusions, transcript isoforms, cSNPs, and allele-specific expression.

Targeted RNA sequencing is a highly accurate and specific method for measuring expression of transcripts of interest, offering researchers both quantitative and qualitative information. This allows for differential expression analysis, as well as allele-specific expression measurement and verification of the presence of fusion genes.

Targeted cancer sequencing with FFPE-compatible RNA panels allows researchers to study gene expression changes and gene fusions, providing a focused view of the functionally relevant changes occurring in cancer.